Optimization of ethanol reflux extraction process of Ziziphi Spinosae Semen- Schisandrae Sphenantherae Fructus based on network pharmacology combined with response surface methodology.
10.19540/j.cnki.cjcmm.20220712.303
- Author:
Mian HUANG
1
;
Yu-Meng SONG
1
;
Xi-Yue WANG
1
;
Bing-Tao ZHAI
1
;
Jiang-Xue CHENG
1
;
Xiao-Fei ZHANG
1
;
Dong-Yan GUO
1
Author Information
1. State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources (Cultivation), Shaanxi Key Laboratory of Chinese Medicine Fundamentals and New Drugs Research, Shaanxi University of Chinese Medicine Xianyang 712046, China.
- Publication Type:Journal Article
- Keywords:
Box-Behnken method;
Schisandrae Sphenantherae Fructus;
Ziziphi Spinosae Semen;
extraction process;
network pharmacology
- MeSH:
Ethanol;
Molecular Docking Simulation;
Network Pharmacology;
Seeds/chemistry*;
Ziziphus/chemistry*;
Plant Extracts/chemistry*;
Schisandra/chemistry*;
Fruit/chemistry*;
Technology, Pharmaceutical
- From:
China Journal of Chinese Materia Medica
2023;48(4):966-977
- CountryChina
- Language:Chinese
-
Abstract:
The present study optimized the ethanol extraction process of Ziziphi Spinosae Semen-Schisandrae Sphenantherae Fructus drug pair by network pharmacology and Box-Behnken method. Network pharmacology and molecular docking were used to screen out and verify the potential active components of Ziziphi Spinosae Semen-Schisandrae Sphenantherae Fructus, and the process evaluation indexes were determined in light of the components of the content determination under Ziziphi Spinosae Semen and Schisandrae Sphenantherae Fructus in the Chinese Pharmacopoeia(2020 edition). The analytic hierarchy process(AHP) was used to determine the weight coefficient of each component, and the comprehensive score was calculated as the process evaluation index. The ethanol extraction process of Ziziphi Spinosae Semen-Schisandrae Sphenantherae Fructus was optimized by the Box-Behnken method. The core components of the Ziziphi Spinosae Semen-Schisandrae Sphenantherae Fructus drug pair were screened out as spinosin, jujuboside A, jujuboside B, schisandrin, schisandrol, schisandrin A, and schisandrin B. The optimal extraction conditions obtained by using the Box-Behnken method were listed below: extraction time of 90 min, ethanol volume fraction of 85%, and two times of extraction. Through network pharmacology and molecular docking, the process evaluation indexes were determined, and the optimized process was stable, which could provide an experimental basis for the production of preparations containing Ziziphi Spinosae Semen-Schisandrae Sphenantherae Fructus.
