Application of partial least squares algorithm to explore bioactive components of crude and stir-baked hawthorn for invigorating spleen and promoting digestion.
10.19540/j.cnki.cjcmm.20220712.302
- Author:
Fei SUN
1
;
Xiang-Qin WU
2
;
Yue QI
2
;
Xing-Yu CHEN
2
;
Yu-Hua CAO
3
;
Jian-Gang WANG
3
;
Shu-Mei WANG
1
;
Sheng-Wang LIANG
1
Author Information
1. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine Guangzhou 510006, China Engineering Technology Research Center for Chinese Materia Medica Quality of Guangdong Province Guangzhou 510006, China.
2. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China.
3. Shandong Bokang Traditional Chinese Medicine Co., Ltd. Weifang 262500, China.
- Publication Type:Journal Article
- Keywords:
bioactive components;
hawthorn;
invigorating spleen and promoting digestion;
partial least squares;
processing mechanism;
spectrum-effect relationship;
stir-baked hawthorn
- MeSH:
Animals;
Rats;
Spleen;
Crataegus;
Quinic Acid;
Least-Squares Analysis;
Vanillic Acid;
Algorithms;
Digestion
- From:
China Journal of Chinese Materia Medica
2023;48(4):958-965
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at identifying the bioactive components of the crude and stir-baked hawthorn for invigorating spleen and promoting digestion, respectively, to clarify the processing mechanism of hawthorn by applying the partial least squares(PLS) algorithm to build the spectrum-effect relationship model. Firstly, different polar fractions of crude and stir-baked hawthorn aqueous extracts and combinations of different fractions were prepared, respectively. Then, the contents of 24 chemical components were determined by ultra-high performance liquid chromatography-mass spectrometry. The effects of different polar fractions of crude hawthorn and stir-baked hawthorn aqueous extracts and combinations of different fractions were evaluated by measuring the gastric emptying rate and small intestinal propulsion rate. Finally, the PLS algorithm was used to establish the spectrum-effect relationship model. The results showed that there were significant differences in the contents of 24 chemical components for different polar fractions of crude and stir-baked hawthorn aqueous extracts and combinations of different fractions, and the gastric emptying rate and small intestinal propulsion rate of model rats were improved by administration of different polar fractions of crude and stir-baked hawthorn aqueous extracts and combinations of different fractions. The bioactive components of crude hawthorn identified by PLS models were vitexin-4″-O-glucoside, vitexin-2″-O-rhamnoside, neochlorogenic acid, rutin, gallic acid, vanillic acid, citric acid, malic acid, quinic acid and fumaric acid, while neochlorogenic acid, cryptochlorogenic acid, rutin, gallic acid, vanillic acid, citric acid, quinic acid and fumaric acid were the bioactive components of stir-baked hawthorn. This study provided data support and scientific basis for identifying the bioactive components of crude and stir-baked hawthorn, and clarifying the processing mechanism of hawthorn.
