Mechanism of Xiaoer Chiqiao Qingre Granules in clearing heat and removing food stagnation in suckling rats with fever and food accumulation based on metabolomics.
10.19540/j.cnki.cjcmm.20220830.502
- Author:
Meng-Yao CUI
1
;
Yan ZHANG
2
;
Nan SI
2
;
Yan-Yan ZHOU
2
;
Kun WANG
3
;
Hong-Jie WANG
2
;
Geng LI
4
;
Li-Qi NI
3
;
Hai-Yu ZHAO
2
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Jiangxi University of Chinese Medicine Nanchang 300043, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Jumpcan Pharmaceutical Group Co., Ltd. Taizhou 225400, China.
4. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
Xiaoer Chiqiao Qingre Granules;
clearing heat and removing food stagnation;
lipid peroxide;
metabolomics;
tricarboxylic acid cycle
- MeSH:
Animals;
Rats;
Rats, Sprague-Dawley;
Hot Temperature;
Tandem Mass Spectrometry;
Metabolomics;
Food;
Fever;
Interferon-gamma
- From:
China Journal of Chinese Materia Medica
2023;48(3):811-822
- CountryChina
- Language:Chinese
-
Abstract:
Children's fever is often accompanied by food accumulation. Traditional Chinese medicine believes that removing food stagnation while clearing heat of children can effectively avoid heat damage. To systematically evaluate the efficacy of Xiaoer Chiqiao Qingre Granules(XRCQ) in clearing heat and removing food accumulation and explore its potential mechanism, this study combined suckling SD rats fed with high-sugar and high-fat diet with injection of carrageenan to induce rat model of fever and food accumulation. This study provided references for the study on the pharmacodynamics and mechanism of XRCQ. The results showed that XRCQ effectively reduced the rectal temperature of suckling rats, improved the inflammatory environment such as the content of interleukin-1β(IL-1β), interleukin-2(IL-2), interferon-γ(IFN-γ), white blood cells, and monocytes. XRCQ also effectively repaired intestinal injury and enhanced intestinal propulsion function. According to the confirmation of its efficacy of clearing heat, the thermolytic mechanism of XRCQ was further explored by non-targeted and targeted metabolomics methods based on LTQ-Orbitrap MS/MS and UPLC-QQQ-MS/MS. Non-target metabolomics analysis of brain tissue samples was performed by QI software combined with SIMCA-P software, and 22 endogenous metabolites that could be significantly regulated were screened out. MetaboAnalyst pathway enrichment results showed that the intervention mechanism was mainly focused on tyrosine metabolism, tricarboxylic acid cycle, inositol phosphate metabolism, and other pathways. At the same time, the results of targeted metabolomics of brain tissue samples showed that XRCQ changed the vitality of digestive system, and inhibited abnormal energy metabolism and inflammatory response, playing a role in clearing heat and removing food stagnation from multiple levels.
