Mechanism of Berberis atrocarpa anthocyanin against Alzheimer's disease based on network pharmacology and experimental verification.
10.19540/j.cnki.cjcmm.20220905.706
- Author:
Hai-Yan BAO
1
;
Ling CHEN
1
;
Ying YANG
1
;
Min LI
1
;
Hui-Min LI
1
;
Ying-Ying KANG
1
;
Jian-Guang LI
2
Author Information
1. College of Pharmacy, Xinjiang Medical University Urumqi 830011, China.
2. College of Pharmacy, Xinjiang Medical University Urumqi 830011, China Xinjiang University of Science and Technology Korla 841000, China.
- Publication Type:Journal Article
- Keywords:
Alzheimer′s disease;
Berberis atrocarpa;
NF-κB/TLR4 signal pathway;
anthocyanin;
molecular docking;
network pharmacology
- MeSH:
NF-kappa B;
Alzheimer Disease;
Network Pharmacology;
Anthocyanins;
Berberis;
Lipopolysaccharides;
Molecular Docking Simulation;
Myeloid Differentiation Factor 88;
Neuroinflammatory Diseases;
Toll-Like Receptor 4;
I-kappa B Proteins
- From:
China Journal of Chinese Materia Medica
2023;48(3):778-788
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to explore the potential mechanism of Berberis atrocarpa Schneid. anthocyanin against Alzheimer's disease(AD) based on network pharmacology, molecular docking technology, and in vitro experiments. Databases were used to screen out the potential targets of the active components of B. atrocarpa and the targets related to AD. STRING database and Cytoscape 3.9.0 were adopted to construct a protein-protein interaction(PPI) network and carry out topological analysis of the common targets. Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analyses were performed on the target using the DAVID 6.8 database. Molecular docking was conducted to the active components and targets related to the nuclear factor kappa B(NF-κB)/Toll-like receptor 4(TLR4) pathway. Finally, lipopolysaccharide(LPS) was used to induce BV2 cells to establish the model of AD neuroinflammation for in vitro experimental validation. In this study, 426 potential targets of active components of B. atrocarpa and 329 drug-disease common targets were obtained, and 14 key targets were screened out by PPI network. A total of 623 items and 112 items were obtained by GO functional enrichment analysis and KEGG pathway enrichment analysis, respectively. Molecular docking results showed that NF-κB, NF-κB inhibitor(IκB), TLR4, and myeloid differentiation primary response 88(MyD88) had good binding abilities to the active components, and malvidin-3-O-glucoside had the strongest binding ability. Compared with the model group, the concentration of nitric oxide(NO) decreased at different doses of malvidin-3-O-glucoside without affecting the cell survival rate. Meanwhile, malvidin-3-O-glucoside down-regulated the protein expressions of NF-κB, IκB, TLR4, and MyD88. This study uses network pharmacology and experimental verification to preliminarily reveal that B. atrocarpa anthocyanin can inhibit LPS-induced neuroinflammation by regulating the NF-κB/TLR4 signaling pathway, thereby achieving the effect against AD, which provides a theoretical basis for the study of its pharmacodynamic material basis and mechanism.
