Astragali Radix-Curcumae Rhizoma combination inhibits proliferation, migration, and invasion of colon cancer HT-29 cells by regulating EMT.
10.19540/j.cnki.cjcmm.20221102.701
- Author:
Qi YANG
1
;
Zheng SUN
2
;
Yi-Miao ZHU
1
;
Dong-Yang XIANG
1
;
Qun-Yao ZHANG
1
;
Fang WANG
1
;
Gang YANG
1
;
Hao YANG
1
;
De-Cai TANG
3
;
Xiao-Yu WU
2
Author Information
1. the First School of Clinical Medicine, Nanjing University of Chinese Medicine Nanjing 210023, China.
2. Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 210004, China.
3. School of Chinese Medicine·School of Integrated Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210023, China.
- Publication Type:Journal Article
- Keywords:
Astragali Radix-Curcumae Rhizoma;
colon cancer;
epithelial-mesenchymal transition(EMT);
invasion and migration;
proliferation
- MeSH:
Humans;
Animals;
Mice;
Caspase 3;
Matrix Metalloproteinase 2;
Matrix Metalloproteinase 9;
Vimentin;
HT29 Cells;
bcl-2-Associated X Protein;
Colonic Neoplasms;
Cell Proliferation
- From:
China Journal of Chinese Materia Medica
2023;48(3):736-743
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the effect of Astragali Radix-Curcumae Rhizoma(AC) combination on the proliferation, migration, and invasion of colon cancer HT-29 cells based on epithelial-mesenchymal transition(EMT). HT-29 cells were respectively treated with 0, 3, 6 and 12 g·kg~(-1) AC-containing serum for 48 h. The survival and growth of cells were measured by thiazole blue(MTT) colorimetry, and the proliferation, migration, and invasion of cells were detected by 5-ethynyl-2'-deoxyuridine(EdU) test and Transwell assay. Cell apoptosis was examined by flow cytometry. The BALB/c nude mouse model of subcutaneous colon cancer xenograft was established, and then model mice were classified into blank control group, 6 g·kg~(-1) AC group, and 12 g·kg~(-1) AC group. The tumor weight and volume of mice were recorded, and the histopathological morphology of the tumor was observed based on hematoxylin-eosin(HE) staining. The expression of apoptosis-associated proteins B-cell lymphoma-2-associated X protein(Bax), cysteine-aspartic acid protease-3(caspase-3), and cleaved caspase-3, and EMT-associated proteins E-cadherin, MMP9, MMP2 and vimentin in HT-29 cells and mouse tumor tissues after the treatment of AC was determined by Western blot. The results showed that cell survival rate and the number of cells at proliferation stage decreased compared with those in the blank control group. The number of migrating and invading cells reduced and the number of apoptotic cells increased in the administration groups compared with those in the blank control group. As for the in vivo experiment, compared with the blank control group, the administration groups had small tumors with low mass and shrinkage of cells and karyopycnosis in the tumor tissue, indicating that the AC combination may improve EMT. In addition, the expression of Bcl2 and E-cadherin increased and the expression of Bax, caspase-3, cleaved caspase-3, MMP9, MMP2, and vimentin decreased in HT-29 cells and tumor tissues in each administration group. In summary, the AC combination can significantly inhibit the proliferation, invasion, migration, and EMT of HT-29 cells in vivo and in vitro and promote the apoptosis of colon cancer cells.
