Analysis and evaluation on Paeoniae Radix Alba from different cultivars by UPLC-Q-TOF-MS and HPLC.
10.19540/j.cnki.cjcmm.20220618.201
- Author:
Shuang YANG
1
;
Qian-Qian DU
1
;
Qian-Xia YUE
1
;
Ye-Fen SUN
1
;
Chuan-Shan JIN
2
;
Wei ZHANG
1
;
Lei MA
3
;
Cong-Bin LIU
1
;
Dong-Mei XIE
2
Author Information
1. Anhui University of Chinese Medicine Hefei 230012, China.
2. Anhui University of Chinese Medicine Hefei 230012, China Institute of Chinese Medicine Resources Protection and Development, Anhui Academy of Traditional Chinese Medicine Hefei 230012, China.
3. Anhui Jiren Pharmaceutical Co.,Ltd. Bozhou 236800, China.
- Publication Type:Journal Article
- Keywords:
HPLC;
Paeoniae Radix Alba;
UPLC-Q-TOF-MS;
cultivars;
quality evaluation
- MeSH:
Chromatography, High Pressure Liquid;
Paeonia;
Acetonitriles
- From:
China Journal of Chinese Materia Medica
2023;48(3):715-724
- CountryChina
- Language:Chinese
-
Abstract:
In this study, an established ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS) method was combined with multivariate statistical analysis to investigate the commonality and difference of main chemical components in the medicinal parts of Paeonia lactiflora from different cultivars; in addition, a high performance liquid chromatography(HPLC) method was established to simultaneously determine the content of eight active components in Paeoniae Radix Alba. Non-targeted analysis was carried out by UPLC-Q-TOF-MS on a Waters ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm, 1.7 μm) column with a gradient elution of 0.1% aqueous formic acid(A)-acetonitrile(B) as the mobile phase at a flow rate of 0.2 mL·min~(-1). The column temperature was 30 ℃, and an electrospray ionization source was used to acquire mass spectrometry data in positive and negative ion modes. According to the accurate molecular weight and fragment ion information provided by multi-stage mass spectrometry and by comparison with reference substances and literature reports, thirty-six identical components were identified in Paeoniae Radix Alba from different cultivars with positive and negative ion modes. In the negative ion mode, two groups of samples were well separated; specifically, seventeen components with significant differences in content were screened and identified, and one component unique in "Bobaishao" was obtained. Quantitative analysis was conducted by high-performance liquid chromatography(HPLC) on an Agilent HC-C_(18)(4.6 mm×250 mm, 5 μm) column with a gradient elution of 0.1% aqueous phosphoric acid(A)-acetonitrile(B) as the mobile phase at a flow rate of 1.0 mL·min~(-1). The column temperature was 30 ℃ and the detection wavelength was at 230 nm. An HPLC method was developed for the simultaneous determination of eight active components(gallic acid, oxypaeoniflorin, catechin, albiflorin, paeoniflorin, galloylpaeoniflorin, 1,2,3,4,6-O-pentagalloylglucose, benzoyl-paeoniflorin) in Paeoniae Radix Albaa from different cultivars. Satisfactory linearity was achieved within the investigated linear ranges and with fine coefficients(r>0.999 0), and the methodological investigation showed that the method had good precision, repeatability and stability. The mean recoveries were 90.61% to 101.7% with RSD of 0.12% to 3.6%(n=6). UPLC-Q-OF-MS provided a rapid and efficient qualitative analytical method for the identification of the chemical components in Paeoniae Radix Alba, and the developed HPLC method was simple, rapid and accurate, which could provide a scientific basis for the evaluation of the germplasm resources and herbal quality of Paeoniae Radix Alba from different cultivars.
