Preparation of two tanshinone Ⅱ_A-astragaloside Ⅳ co-loaded nano-delivery systems and in vitro antitumor activity comparison.
10.19540/j.cnki.cjcmm.20221127.302
- Author:
Xia LI
1
;
Hong GUO
1
;
Deng-Xuan MAO
1
;
Yu-Ping LIU
1
;
Yan CHEN
1
Author Information
1. Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine Nanjing 210028, China Multi-component of Traditional Chinese Medicine and Microecology Research Center,Jiangsu Provincial Academy of Traditional Chinese Medicine Nanjing 210028, China.
- Publication Type:Journal Article
- Keywords:
antitumor;
astragaloside Ⅳ;
metal-organic frameworks(MOF);
microemulsion;
nano-drug delivery;
tanshinone Ⅱ_A
- MeSH:
Mice;
Animals;
Endothelial Cells;
Abietanes;
Cell Line
- From:
China Journal of Chinese Materia Medica
2023;48(3):672-680
- CountryChina
- Language:Chinese
-
Abstract:
This study screened excellent carriers for co-loading tanshinone Ⅱ_A(TSA) and astragaloside Ⅳ(As) to construct antitumor nano-drug delivery systems for TSA and As. TSA-As microemulsions(TSA-As-MEs) were prepared by water titration. TSA-As metal-organic framework(MOF) nano-delivery system was prepared by loading TSA and As in MOF by the hydrothermal method. Dynamic light scattering(DLS), transmission electron microscopy(TEM), and scanning electron microscopy(SEM) were used to characterize the physicochemical properties of the two preparations. Drug loading was determined by HPLC and the effects of the two preparations on the proliferation of vascular endothelial cells, T lymphocytes, and hepatocellular carcinoma cells were detected by the CCK-8 method. The results showed that the particle size, Zeta potential, and drug loading of TSA-As-MEs were(47.69±0.71) nm,(-14.70±0.49) mV, and(0.22±0.01)%, while those of TSA-As-MOF were(258.3±25.2) nm,(-42.30 ± 1.27) mV, and 15.35%±0.01%. TSA-As-MOF was superior to TSA-As-MEs in drug loading, which could inhibit the proliferation of bEnd.3 cells at a lower concentration and improve the proliferation ability of CTLL-2 cells significantly. Therefore, MOF was preferred as an excellent carrier for TSA and As co-loading.
