Effect of sulfur fumigation on quality and safety of Lilii Bulbus.
10.19540/j.cnki.cjcmm.20221031.301
- Author:
Rong ZHANG
1
;
Ya-Ling YANG
2
;
Ai-Ping DENG
3
;
Li-Ping KANG
2
;
Ming-En CHENG
4
;
Chuan-Zhi KANG
5
;
Lan-Ping GUO
5
Author Information
1. School of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China State Key Laboratory Base of Daodi Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. State Key Laboratory Base of Daodi Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Hubei University of Chinese Medicine Wuhan 430065, China.
4. School of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China.
5. State Key Laboratory Base of Daodi Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Key Laboratory of Biology and Cultivation of Herb Medicine, Ministry of Agriculture and Rural Affairs Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Lilii Bulbus;
UPLC-Q-TOF-MS/MS;
cytotoxicity;
markers of sulfur fumigation;
sulfur fumigation
- MeSH:
Humans;
Animals;
Rats;
Fumigation;
Tandem Mass Spectrometry;
Chromatography, High Pressure Liquid;
Epithelial Cells;
Sulfur
- From:
China Journal of Chinese Materia Medica
2023;48(3):660-671
- CountryChina
- Language:Chinese
-
Abstract:
Lilii Bulbus is a commonly used Chinese herbal medicine with both medicinal and edible values, while the market products usually has the problem of sulfur fumigation. Therefore, the quality and safety of Lilii Bulbus products deserve attention. In this study, ultra-high performance liquid chromatography-time of flight-tandem mass spectrometry(UPLC-Q-TOF-MS/MS) was combined with principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA) to analyze the differential components of Lilii Bulbus before and after sulfur fumigation. We identified ten markers generated after sulfur fumigation, summarized their mass fragmentation and transformation patterns, and verified the structures of phenylacrylic acid markers of sulfur fumigation. At the same time, the cytotoxicity of the aqueous extracts of Lilii Bulbus before and after sulfur fumigation was evaluated. The results showed that in the concentration range of 0-800 mg·L~(-1), the aqueous extract of Lilii Bulbus after sulfur fumigation had no significant effect on the viability of human liver LO2 cells, human renal proximal tubular HK-2 cells, and rat adrenal pheochromocytoma PC-12 cells. Moreover, the viability of the cells exposed to the aqueous extract of Lilii Bulbus before and after sulfur fumigation showed no significant difference. This study identified phenylacrylic acid and furostanol saponins as markers of sulfur-fumigated Lilii Bulbus for the first time, and made clear that proper sulfur fumigation of Lilii Bulbus would not produce cytotoxicity, providing a theoretical basis for the rapid identification and quality and safety control of sulfur-fumigated Lilii Bulbus.
