Anti-depression mechanism of Zuojin Pills:based on UHPLC-TOF-MS, network pharmacology, and experimental verification.
10.19540/j.cnki.cjcmm.20221102.706
- Author:
Guo-Liang DAI
1
;
Hua-Xi HANG
1
;
Pei-Yao CHEN
1
;
Sheng-Wei HONG
1
;
Mei-Juan XU
1
;
Cheng-Yao MA
1
;
Qian HUANG
1
;
Yu YE
1
;
Mei-Shuang YU
1
;
Wen-Zheng JU
1
Author Information
1. Department of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 210029, China.
- Publication Type:Journal Article
- Keywords:
UHPLC-TOF-MS;
Zuojin Pills;
depression;
mice;
network pharmacology
- MeSH:
Animals;
Mice;
Network Pharmacology;
AMP-Activated Protein Kinases;
Chromatography, High Pressure Liquid;
NLR Family, Pyrin Domain-Containing 3 Protein;
Sirtuin 1;
Drugs, Chinese Herbal/pharmacology*;
Molecular Docking Simulation
- From:
China Journal of Chinese Materia Medica
2023;48(1):183-192
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to explore the anti-depression mechanism of Zuojin Pills based on the plasma constituents, network pharmacology, and experimental verification. UHPLC-TOF-MS was used for qualitative analysis of Zuojin Pills-containing serum. Targets of the plasma constituents and the disease were retrieved from PharmMapper and GeneCards. Then the protein-protein interaction(PPI) network was constructed and core targets were screened for GO term enrichment and KEGG pathway enrichment. Cytoscape 3.7.2 was employed construct the "compound-target-pathway" network and the targets and signaling pathways of Zuojin Pills against depression were predicted. CUMS-induced depression mouse model was established to verify the key targets. The results showed that a total of 21 constituents migrating to blood of Zuojin Pills were identified, which were mainly alkaloids. A total of 155 common targets of the constituents and the disease and 67 core targets were screened out. KEGG enrichment and PPI network analysis showed that Zuojin Pills may play a role in the treatment of depression through AMPK/SIRT1, NLRP3, insulin and other targets and pathways. Furthermore, the results of animal experiments showed that Zuojin Pills could significantly improve the depression behaviors of depression, reduce the levels of IL-1β, IL-6 and TNF-α in hippocampus and serum, activate AMPK/SIRT1 signaling, and reduce the protein expression of NLRP3. In conclusion, Zuojin Pills may play a role in the treatment of depression by activating AMPK/SIRT1 signaling pathway, and inhibiting NLRP3 activation and neuroinflammation in the hippocampus of mice.
