Chemical constituents from fruits of Morinda citrifolia and their inhibitory effects on proliferation of synoviocytes in vitro.
10.19540/j.cnki.cjcmm.20220421.203
- Author:
Zhang-Yang SHEN
1
;
Ruo-Qing GUAN
1
;
Meng-Ran DU
1
;
Yuan BIAN
1
;
Yu WANG
1
;
Xin-Yuan SUO
1
;
Shu-Hong XIONG
1
;
Yan-Ping LIU
2
;
Yan-Hui FU
2
Author Information
1. Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,Hainan Normal University Haikou 571127,China.
2. Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,Hainan Normal University Haikou 571127,China Engineering Research Center for Industrialization of Southern Medicinal Plants Resources of Hainan Province,Hainan Normal University Haikou 571127,China Key Laboratory of Research and Development of Tropical Fruits and Vegetables of Haikou City,Hainan Normal University Haikou 571127,China.
- Publication Type:Journal Article
- Keywords:
alkaloids;
fruits of Morinda citrifolia;
inhibitory effects on the proliferation of synoviocytes;
lignans;
phenolic acids;
pyrone derivative
- MeSH:
Fruit/chemistry*;
Morinda/chemistry*;
Synoviocytes;
Cell Proliferation;
Arthritis
- From:
China Journal of Chinese Materia Medica
2023;48(1):105-113
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents from the fruits of Morinda citrifolia were systematically explored by chromatographic fractionation methods including silica gel, octadecylsilyl(ODS) gel, Sephadex LH-20 gel, and preparative high performance liquid chromatography(pre-HPLC). The chemical structures of all isolated compounds were identified on the basis of their physicochemical properties, spectroscopic analyses, as well as the comparisons of their physicochemical and spectroscopic data with the reported data in literature. As a result, 22 isolated compounds from the 90% ethanol extract of the fruits of M. citrifolia were identified, which were moricitritone(1), 2'-deoxythymidine(2), cyclo-(L-Pro-L-Tyr)(3), methyl-5-hydroxy-2-pyridinecarboxylate(4), methyl pyroglutamate(5), bisbenzopyran(6), epipinoresinol(7), 3, 3'-bisdemethyl pinoresinol(8), 3, 3'-bisdemethyltanegool(9), trimesic acid(10), crypticin B(11), kojic acid(12), vanillic acid(13), protocatechoic acid(14), 5-hydroxymethyl furfural(15), blumenol A(16), 1-O-(9Z, 12Z-octadecadienoyl) glycerol(17), mucic acid dimethylester(18), methyl 2-O-β-D-glucopyranosylbenzoate(19), 2-phenylethyl-O-β-D-glucoside(20), scopoletin(21), and quercetin(22). Among them, compound 1 was a new pyrone derivative, compounds 2, 4-7, 10-12, and 17 were isolated from the plants belonging to Morinda genus for the first time, and compound 18 was obtained from M. citrifolia for the first time. Moreover, on the basis of testing the activities of all isolated compounds on inhibiting the proliferation of synovial fibroblasts in vitro by MTS assay, the anti-rheumatoid arthritis activities of all isolated compounds were initially evaluated. The results showed that compounds 1-6, 9, 19, and 20 exhibited remarkable anti-rheumatoid arthritis activities, which displayed the inhibitory effects on the proliferation of MH7A synovial fibroblast cells with the IC_(50) values in the range of(3.69±0.08) to(168.96±0.98) μmol·L~(-1).
