Identification and biological characteristics of violet root rot pathogen of Pseudostellaria heterophylla in Fujian province.
10.19540/j.cnki.cjcmm.20221016101
- Author:
Bao-Cai LIU
1
;
Jing-Ying CHEN
1
;
Wu-Jun ZHANG
1
;
Ying-Zhen HUANG
1
;
Yun-Qing ZHAO
1
;
Jing YE
2
Author Information
1. Agricultural Bioresource Institute, Fujian Academy of Agricultural Sciences Fuzhou 350003, China Research Center for Medicinal Plant, Fujian Academy of Agricultural Sciences Fuzhou 350003, China.
2. Research Center for Medicinal Plant, Fujian Academy of Agricultural Sciences Fuzhou 350003, China.
- Publication Type:Journal Article
- Keywords:
Helicobasidium mompa;
Pseudostellaria heterophylla;
biological characteristics;
pathogen identification;
violet root rot
- MeSH:
Plant Roots;
Phylogeny;
Temperature;
Caryophyllaceae;
Nitrogen
- From:
China Journal of Chinese Materia Medica
2023;48(1):45-51
- CountryChina
- Language:Chinese
-
Abstract:
Violet root rot is one of the main root diseases in the production process of Pseudostellaria heterophylla. To clarify the pathogenic species that cause the violet root rot of P. heterophylla in Fujian province, the roots and the sclerotia with violet root rot symptoms were collected from the main producing areas of P. heterophylla(Fujian province) from 2017 to 2021, and the pathogens were isolated by tissue separation method and identified by morphology and multi-gene phylogenetic analysis. Additionally, the biological characteristics of the pathogens were studied and the fungicides were determined. The results showed that 78 strains of violet root rot were isolated from the collected root samples, which belonged to one type after preliminary morphological identification. Two represen-tative strains were selected from the pathogens for multi-gene phylogenetic analysis, and they were clustered with Helicobasidium mompa together. The suitable culture conditions for the mycelium were OA medium, 25 ℃, pH 6, and ammonium oxalate as the nitrogen source. The lethal temperature of the mycelium was 50 ℃ for 10 minutes. Moreover, 99.1% propiconazole and 98.7% azoxystrobin had the optimal bacteriostatic effect, and the concentrations with the 50% bacteriostatic rate were 16.85 and 12.24 μg·mL~(-1), respectively. On the basis of the above results, the pathogen causing violet root rot of P. heterophylla in Fujian province was H. mompa. The medium type, growth temperature, pH value, nitrogen source, etc. had significant effect on the growth of mycelium.
