Effective substances and mechanism of Yishen Guluo Mixture in treatment of chronic glomerulonephritis based on metabolomics and serum pharmacochemistry.
10.19540/j.cnki.cjcmm.20220905.705
- Author:
Zhen-Hua BIAN
1
;
Wen-Ming ZHANG
1
;
Jing-Yue TANG
1
;
Qian-Qian FEI
1
;
Min-Min HU
1
;
Xiao-Wei CHEN
1
;
Xiao-Hang YUAN
1
;
Tu-Lin LU
2
Author Information
1. Department of Pharmacy, Wuxi Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine Wuxi 214071, China.
2. College of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.
- Publication Type:Journal Article
- Keywords:
Yishen Guluo Mixture;
biomarkers;
chronic glomerulonephritis;
components absorbed into blood;
effective substance;
mechanism of action;
metabolomics
- MeSH:
Animals;
Rats;
Arachidonic Acid;
Biomarkers/blood*;
Blood Proteins;
Chromatography, High Pressure Liquid;
Creatinine;
Drugs, Chinese Herbal/therapeutic use*;
Glomerulonephritis/metabolism*;
Metabolomics;
Urea;
Chronic Disease;
Disease Models, Animal;
Complex Mixtures/therapeutic use*
- From:
China Journal of Chinese Materia Medica
2023;48(2):492-506
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to investigate the effective substances and mechanism of Yishen Guluo Mixture in the treatment of chronic glomerulonephritis(CGN) based on metabolomics and serum pharmacochemistry. The rat model of CGN was induced by cationic bovine serum albumin(C-BSA). After intragastric administration of Yishen Guluo Mixture, the biochemical indexes related to renal function(24-hour urinary protein, serum urea nitrogen, and creatinine) were determined, and the efficacy evaluations such as histopathological observation were carried out. The serum biomarkers of Yishen Guluo Mixture in the treatment of CGN were screened out by ultra-performance liquid chromatography-quadrupole time-of-flight/mass spectrometry(UPLC-Q-TOF-MS) combined with multivariate statistical analysis, and the metabolic pathways were analyzed. According to the mass spectrum ion fragment information and metabolic pathway, the components absorbed into the blood(prototypes and metabolites) from Yishen Guluo Mixture were identified and analyzed by using PeakView 1.2 and MetabolitePilot 2.0.4. By integrating metabolomics and serum pharmacochemistry data, a mathematical model of correlation analysis between serum biomarkers and components absorbed into blood was constructed to screen out the potential effective substances of Yishen Guluo Mixture in the treatment of CGN. Yishen Guluo mixture significantly decreased the levels of 24-hour urinary protein, serum urea nitrogen, and creatinine in rats with CGN, and improved the pathological damage of the kidney tissue. Twenty serum biomarkers of Yishen Guluo Mixture in the treatment of CGN, such as arachidonic acid and lysophosphatidylcholine, were screened out, involving arachidonic acid metabolism, glycerol phosphatide metabolism, and other pathways. Based on the serum pharmacochemistry, 8 prototype components and 20 metabolites in the serum-containing Yishen Guluo Mixture were identified. According to the metabolomics and correlation analysis of serum pharmacochemistry, 12 compounds such as genistein absorbed into the blood from Yishen Guluo Mixture were selected as the potential effective substances for the treatment of CGN. Based on metabolomics and serum pharmacochemistry, the effective substances and mechanism of Yishen Guluo Mixture in the treatment of CGN are analyzed and explained in this study, which provides a new idea for the development of innovative traditional Chinese medicine for the treatment of CGN.
