Chemical constituents in different parts of Ixeris sonchifolia based on UPLC-LTQ-Orbitrap-MS~n.
10.19540/j.cnki.cjcmm.20220506.202
- Author:
Yi-Ran REN
1
;
Yuan-Yuan ZHAO
1
;
Zhao-Wei LIU
1
;
Zhen-Qing LIU
2
;
Ying LIU
3
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
2. Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine Beijing 102488, China.
3. Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine Beijing 102488, China Beijing Key Laboratory for Production Process Control and Quality Evaluation of Traditional Chinese Medicine,Beijing Municipal Science & Technology Commission Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
Ixeris sonchifolia;
UPLC-LTQ-Orbitrap-MS~n;
chemical constituent;
different parts of Chinese medicinal materials;
fragmentation pattern
- MeSH:
Drugs, Chinese Herbal/chemistry*;
Methanol;
Chromatography, High Pressure Liquid/methods*;
Mass Spectrometry;
Asteraceae
- From:
China Journal of Chinese Materia Medica
2023;48(2):430-442
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents in stem leaf, root, and flower of Ixeris sonchifolia were identified by the ultra performance li-quid chromatography coupled with linear ion trap quadrupole-orbitrap mass spectrometry(UPLC-LTQ-Orbitrap-MS~n). The separation was performed on an Acquity UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm) with a mobile phase of water(containing 0.1% formic acid, A)-acetonitrile(B) with gradient elution. With electrospray ionization source, the data of 70% methanol extract from stem leaf, root and flower of I. sonchifolia were collected by high-resolution full-scan Fourier transform spectroscopy, data dependent acquisition, precursor ion scan, and selected ion monitoring in the negative and positive ion modes. The compounds were identified based on accurate molecular weight, retention time, fragment ions, comparison with reference standard, Clog P and references. A total of 131 compounds were identified from the 70% methanol extract of I. sonchifolia, including nucleosides, flavonoids, organic acids, terpenoids, and phenylpropanoids, and 119, 110, and 126 compounds were identified from the stem leaf, root and flower of I. sonchifolia, respectively. In addition, isorhamnetin, isorhamnetin-7-O-sambubioside and caffeylshikimic acid were discovered from I. sonchifolia for the first time. This study comprehensively analyzed and compared the chemical constituents in different parts of I. sonchifolia, which facilitated the discovery of effective substances and the development and application of medicinal material resources of I. sonchifolia.
