Key quality attributes of benchmark samples of famous classical formula Kaixin Powder.
10.19540/j.cnki.cjcmm.20220520.301
- Author:
Bing-Xian SHANG
1
;
Zhen-Xia ZHAO
2
;
Qi ZENG
3
;
Jian SU
2
;
Bing XU
3
;
Yong-Li LIU
2
;
Hai-Min LEI
3
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102400, China Hebei Institute for Drug Control Shijiazhuang 050299, China.
2. Hebei Institute for Drug Control Shijiazhuang 050299, China.
3. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102400, China.
- Publication Type:Journal Article
- Keywords:
Kaixin Powder;
benchmark samples;
content determinationn;
famous classical formula;
specific chromatogram
- MeSH:
Powders;
Ginsenosides;
Benchmarking;
Drugs, Chinese Herbal/chemistry*;
Sucrose;
Chromatography, High Pressure Liquid/methods*
- From:
China Journal of Chinese Materia Medica
2023;48(2):382-389
- CountryChina
- Language:Chinese
-
Abstract:
We prepared 15 batches of Kaixin Powder benchmark samples with the decoction pieces of different batches. Further, we established the specific chromatograms and index component content determination method of Kaixin Powder benchmark samples and analyzed the peaks and similarity of the chromatograms. With sibiricose A5, sibiricose A6, polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose, ginsenoside Rb_1, β-asarone, α-asarone, and dehydropachymic acid as index components, the index component content determination method was established and 70%-130% of the mean content of each component was set as the range. The chromatograms of 15 batches of Kaixin Powder benchmark samples had a total of 22 characteristic peaks, among which 8 peaks were identified, which represented sibiricose A5, sibiricose A6, polygalaxanthone Ⅲ, 3,6'-disinapoyl sucrose, ginsenoside Rb_1, β-asarone, α-asarone, and dehydropachymic acid, respectively. The chromatograms shared the similarity of 0.992-0.999. The 15 batches of benchmark samples had sibiricose A5 of 0.34-0.55 mg·g~(-1), sibiricose A6 of 0.43-0.57 mg·g~(-1), polygalaxanthone Ⅲ of 0.12-0.19 mg·g~(-1), 3,6'-disinapoyl sucrose of 1.08-1.78 mg·g~(-1), ginsenoside Rb_1 of 0.33-0.62 mg·g~(-1), β-asarone of 2.34-3.72 mg·g~(-1), α-asarone of 0.11-0.22 mg·g~(-1), and dehydropachymic acid of 0.053-0.079 mg·g~(-1). This study established the specific chromatograms and index component content determination method of Kaixin Powder benchmark samples, and the method was simple, feasible, reproducible, and stable. This study provides a scientific basis for further research on the key chemical properties of the benchmark samples and preparations of Kaixin Powder.
