Determination of 10 mycotoxins in Hippophae Fructus medicinal and edible products by ultra-performance liquid chromatography- tandem mass spectrometry.
10.19540/j.cnki.cjcmm.20220917.102
- Author:
Xing-Jing YANG
1
;
Yan-Ru LIU
1
;
Zhi-Shu TANG
2
;
Zhong-Xing SONG
1
;
Bai-Jin CHANG
3
;
Yan-Ting ZHAO
1
;
Chang-le LIU
1
Author Information
1. Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, College of Pharmacy, Shaanxi University of Chinese Medicine Xianyang 712083, China.
2. Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, College of Pharmacy, Shaanxi University of Chinese Medicine Xianyang 712083, China China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Changchun University of Chinese Medicine Changchun 130117, China.
- Publication Type:Journal Article
- Keywords:
Hippophae Fructus;
food;
limit analysis;
mycotoxins;
ultra-performance liquid chromatography-tandem mass spectro-metry(UPLC-MS/MS)
- MeSH:
Mycotoxins/analysis*;
Chromatography, Liquid/methods*;
Tandem Mass Spectrometry/methods*;
Hippophae;
Limit of Detection;
Chromatography, High Pressure Liquid/methods*
- From:
China Journal of Chinese Materia Medica
2023;48(2):366-373
- CountryChina
- Language:Chinese
-
Abstract:
An analytical method for 10 mycotoxins in Hippophae Fructus medicinal and edible products was established in this study, and the contamination of their mycotoxins was analyzed. First of all, the mixed reference solution of ten mycotoxins such as aflatoxin, ochratoxin, zearalenone, and dexoynivalenol was selected as the control, and the Hippophae Fructus medicinal and edible products were prepared. Secondly, based on the ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) technology, 10 mycotoxins in Hippophae Fructus medicinal and edible products were quantitatively investigated and their content was determined. Finally, the contamination of mycotoxins was analyzed and evaluated. The optimal analysis conditions were determined, and the methodological inspection results showed that the 10 mycotoxins established a good linear relationship(r>0.99). The method had good repeatability, test sample specificity, stability, and instrument precision. The average recovery rates of 10 mycotoxins in Hippophae Fructus medicinal products, edible solids, and edible liquids were 90.31%-109.4%, 87.86%-107.8%, and 85.61%-109.1%, respectively. Relative standard deviation(RSD) values were 0.22%-10%, 0.75%-13%, and 0.84%-8.5%, repsectively. Based on UPLC-MS/MS technology, the simultaneous determination method for the limits of 10 mycotoxins established in this study has fast detection speed, less matrix interference, high sensitivity, and accurate results, which is suitable for the limit examination of 10 mycoto-xins in Hippophae Fructus medicinal and edible products.
