Effect of Rhizophagus intraradices on growth of Salvia miltiorrhiza.
10.19540/j.cnki.cjcmm.20221017.101
- Author:
Ling-Ling ZHENG
1
;
Mei-Lan CHEN
2
;
Li-Ping KANG
2
;
Ying-Li WANG
3
;
Xiu-Teng ZHOU
2
Author Information
1. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Jiangxi Institute of Traditional Chinese Medicine Health Industry/Institute of Traditional Chinese Medicine Health Industry, China Academy of Chinese Medical Sciences Nanchang 330029, China.
2. State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
3. Baotou City Center for Disease Control and Prevention Baotou 014030, China.
- Publication Type:Journal Article
- Keywords:
Salvia miltiorrhiza;
correlation analysis;
effective components;
endogenous hormones;
growth
- MeSH:
Salvia miltiorrhiza/chemistry*;
Plant Growth Regulators/analysis*;
Plant Roots/chemistry*
- From:
China Journal of Chinese Materia Medica
2023;48(2):349-355
- CountryChina
- Language:Chinese
-
Abstract:
The study aimed to explore the effects of inoculation of Rhizophagus intraradices on the biomass, effective component content, and endogenous hormone content of Salvia miltiorrhiza through pot experiments. The number of leaves, plant height, dry weight of aboveground and underground parts, branch number, root number, root length, root diameter, and other biomass were mea-sured by weighing and counting methods. The content of salvianolic acid B, caffeic acid, rosmarinic acid, tanshinone Ⅰ, tanshinone Ⅱ_A, cryptotanshinone, and other effective components was determined by ultra-high performance liquid chromatography. The content of ABA and GA_3 was determined by triple quadrupole mass spectrometry. The correlations between biomass and effective components and between effective components and plant hormones ABA and GA_3 were analyzed. The results showed that R. intraradices significan-tly increased the aboveground dry weight, leaf number, and root number of S. miltiorrhiza by 0.24-0.65 times, respectively. The content of salvianolic acid B and rosmarinic acid in the aboveground part and the content of salvianolic acid B, caffeic acid, rosmarinic acid, tanshinone Ⅰ, and tanshinone Ⅱ_A in the underground part were significantly increased by 0.44-1.78 times, respectively. R. intraradices infection significantly increased the GA_3/ABA values of aboveground and underground parts by 3.82 and 76.47 times, respectively. The correlation analysis showed that caffeic acid, the effective component of the aboveground part, was significantly positively correlated with plant height, tanshinone Ⅱ_A, the effective component of the underground part, was significantly positively correlated with biomass root number, cryptotanshinone, and dry weight, while rosmarinic acid was significantly negatively correlated with dry weight. There were significant positive correlations between cryptotanshinone and ABA, tanshinone Ⅱ_A and ABA and GA_3, and caffeic acid and GA_3. In conclusion, R. intraradices can promote the accumulation of biomass and secondary metabolites of S. miltiorrhiza and regulate the balance between plant hormones ABA and GA_3, thereby promoting the growth of S. miltiorrhiza.
