Research progress on application of multi-enzyme-catalyzed cascade reactions in enzymatic synthesis of natural products.
10.19540/j.cnki.cjcmm.20221017.102
- Author:
Wen-Qian HUANG
1
;
Ying-Xia WANG
1
;
Wei-Sheng TIAN
1
;
Juan WANG
1
;
Peng-Fei TU
2
;
Xiao-Hui WANG
1
;
She-Po SHI
1
;
Xiao LIU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine Beijing 100029, China.
2. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Beijing 100191, China.
- Publication Type:Journal Article
- Keywords:
biocatalysis;
cascade reactions;
enzyme catalysis;
multi-enzyme catalysis
- MeSH:
Biological Products/chemistry*;
Catalysis;
Alkaloids;
Biocatalysis
- From:
China Journal of Chinese Materia Medica
2023;48(2):336-348
- CountryChina
- Language:Chinese
-
Abstract:
As a biocatalyst, enzyme has the advantages of high catalytic efficiency, strong reaction selectivity, specific target products, mild reaction conditions, and environmental friendliness, and serves as an important tool for the synthesis of complex organic molecules. With the continuous development of gene sequencing technology, molecular biology, genetic manipulation, and other technologies, the diversity of enzymes increases steadily and the reactions that can be catalyzed are also gradually diversified. In the process of enzyme-catalyzed synthesis, the majority of common enzymatic reactions can be achieved by single enzyme catalysis, while many complex reactions often require the participation of two or more enzymes. Therefore, the combination of multiple enzymes together to construct the multi-enzyme cascade reactions has become a research hotspot in the field of biochemistry. Nowadays, the biosynthetic pathways of more natural products with complex structures have been clarified, and secondary metabolic enzymes with novel catalytic activities have been identified, discovered, and combined in enzymatic synthesis of natural/unnatural molecules with diverse structures. This study summarized a series of examples of multi-enzyme-catalyzed cascades and highlighted the application of cascade catalysis methods in the synthesis of carbohydrates, nucleosides, flavonoids, terpenes, alkaloids, and chiral molecules. Furthermore, the existing problems and solutions of multi-enzyme-catalyzed cascade method were discussed, and the future development direction was prospected.
