circ_0092315 Promotes Proliferation and Invasion of Papillary Thyroid Carcinoma Cells via Regulating microRNA-1256/High Mobility Group A2 axis.
10.3881/j.issn.1000-503X.15026
- Author:
Shu-Hong KE
1
;
Cai-Xia KONG
1
;
Yao XU
2
;
Cong PENG
1
Author Information
1. Department of Endocrinology, Wuhan No.1 Hospital,Wuhan 430030,China.
2. Department of Pathology,Wuhan No.1 Hospital,Wuhan 430030,China.
- Publication Type:Journal Article
- Keywords:
circ_0092315;
high mobility group A2;
microRNA-1256;
papillary thyroid carcinoma
- MeSH:
Humans;
Thyroid Cancer, Papillary/genetics*;
Computational Biology;
Thyroid Neoplasms/genetics*;
Cell Proliferation;
MicroRNAs/genetics*
- From:
Acta Academiae Medicinae Sinicae
2023;45(1):16-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and mechanism of circ_0092315 in the proliferation and invasion of papillary thyroid carcinoma cells. Methods The expression of circ_0092315 in papillary thyroid carcinoma cells was examined by real-time fluorescence quantitative PCR.The proliferation and invasion of TPC-1 cells was assessed by CCK-8 and Transwell assays.The protein level of high mobility group A2 (HMGA2) was determined by Western blotting.The regulatory relationship of circ_0092315,microRNA-1256 (miR-1256),and HMGA2 was explored by bioinformatics tools,dual-luciferase reporter assay,real-time fluorescence quantitative PCR,and Western blotting. ++++Results circ_0092315 was overexpressed in papillary thyroid carcinoma cells (all P<0.001).circ_0092315 promoted the proliferation and invasion of TPC-1 cells (all P<0.001).The transfection of si-circ_0092315 up-regulated the expression of miR-1256 (P<0.001),and miR-1256 inhibitor up-regulated the protein level of HMGA2 (P<0.001). ++++Conclusion circ_0092315 is overexpressed in TPC-1 cells and it promotes the proliferation and invasion of TPC-1 cells by regulating the miR-1256/HMGA2 axis.
