Protopanaxadiol-type ginsenoside hydrolases and their application in the preparation of ginsenoside Compound K: a review.

Author: Wenhua YANG ¹ ; Qiuya GU ¹ ; Xiaobin YU ¹
Author Information

1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214000, Jiangsu China.
Publication Type:Journal Article
Keywords: application; enzyme catalysis; ginsenoside Compound K; preparation; protopanaxadiol-type ginsenoside hydrolases
MeSH: Ginsenosides/pharmacology*; Hydrolases; Sapogenins/chemistry*
From: Chinese Journal of Biotechnology 2023;39(3):978-992
CountryChina
Language:Chinese
Abstract: Ginsenoside Compound K (CK) has anti-cancer and anti-inflammatory pharmacological activities. It has not been isolated from natural ginseng and is mainly prepared by deglycosylation of protopanaxadiol. Compared with the traditional physicochemical preparation methods, the preparation of CK by hydrolysis with protopanaxadiol-type (PPD-type) ginsenoside hydrolases has the advantages of high specificity, environmental-friendliness, high efficiency and high stability. In this review, the PPD-type ginsenoside hydrolases were classified into three categories based on the differences in the glycosyl-linked carbon atoms of the hydrolase action. It was found that most of the hydrolases that could prepare CK were PPD-type ginsenoside hydrolase type Ⅲ. In addition, the applications of hydrolases in the preparation of CK were summarized and evaluated to facilitate large-scale preparation of CK and its development in the food and pharmaceutical industries.