Advances in the structure and function of chitosanase.

Author: Jie XIE ¹ ; Yubin LI ¹ ; Jingwei LIU ¹ ; Yan GOU ¹ ; Ganggang WANG ¹
Author Information

1. Key Laboratory of Environmental and Applied Microbiology of Chinese Academy of Sciences, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Publication Type:Journal Article
Keywords: catalytic mechanism; chitosanase; crystal structure; rational design
MeSH: Chitosan/chemistry*; Chitin; Glycoside Hydrolases/genetics*; Protein Engineering; Oligosaccharides/chemistry*; Hydrolysis
From: Chinese Journal of Biotechnology 2023;39(3):912-929
CountryChina
Language:Chinese
Abstract: Chitosanases represent a class of glycoside hydrolases with high catalytic activity on chitosan but nearly no activity on chitin. Chitosanases can convert high molecular weight chitosan into functional chitooligosaccharides with low molecular weight. In recent years, remarkable progress has been made in the research on chitosanases. This review summarizes and discusses its biochemical properties, crystal structures, catalytic mechanisms, and protein engineering, highlighting the preparation of pure chitooligosaccharides by enzymatic hydrolysis. This review may advance the understandings on the mechanism of chitosanases and promote its industrial applications.