α-amylase detection methods and applications.
- Author:
Naying ZHANG
1
;
Guangyu YANG
1
Author Information
- Publication Type:Journal Article
- Keywords: applications; detection methods; α-amylase
- MeSH: Humans; alpha-Amylases/chemistry*; Polysaccharides; Oligosaccharides; Starch; Maltose
- From: Chinese Journal of Biotechnology 2023;39(3):898-911
- CountryChina
- Language:Chinese
- Abstract: α-amylase is an endonucleoside hydrolase that hydrolyzes the α-1, 4-glycosidic bonds inside polysaccharides, such as starch, to generate oligosaccharides, dextrins, maltotriose, maltose and a small amount of glucose. Due to the importance of α-amylase in food industry, human health monitoring and pharmaceuticals, detection of its activity is widely required in the breeding of α-amylase producing strains, in vitro diagnosis, development of diabetes drugs, and the control of food quality. In recent years, many new α-amylase detection methods have been developed with improved speed and sensitivity. This review summarized recent processes in the development and applications of new α-amylase detection methods. The major principle of these detection methods were introduced, and their advantages and disadvantages were compared to facilitate future development and applications of α-amylase detection methods.
