Chromosomal integration of large DNA fragments in microorganisms: a review.

Author: Yuwei WU ¹ ; Weihong JIANG ¹ ; Yang GU ¹
Author Information

1. CAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Publication Type:Journal Article
Keywords: cell factories; chromosomal integration; large DNA fragments; microorganisms
MeSH: Chromosomes; Plasmids; DNA; Cloning, Molecular; Fermentation
From: Chinese Journal of Biotechnology 2023;39(3):842-857
CountryChina
Language:Chinese
Abstract: The modern bio-fermentation industry requires design and creation of efficient microbial cell factories for directed conversion of raw materials to target products. The main criteria for assessing the performance of microbial cell factories are their product synthesis capacity and stability. Due to the deficiencies of plasmids in gene expression such as instability and being easy to lose, integration of genes into chromosome is often a better choice for stable expression in microbial hosts. To this end, chromosomal gene integration technology has received much attention and has developed rapidly. In this review, we summarize the recent research progresses of chromosomal integration of large DNA fragments in microorganisms, illustrate the principles and features of various technologies, highlight the opportunity brought by the CRISPR-associated transposon systems, and prospect future research direction of this technology.