Antagonistic activity and application of Bacillus velezensis strain Bv-303 against rice bacterial-blight disease caused by Xanthomonas oryzae pv. oryzae.
- Author:
Xia LIU
1
;
Zhexiao LU
1
;
Zicheng MA
1
;
Tingting YU
1
;
Haotian CHEN
1
;
Lu WANG
1
;
Xifeng CHEN
1
Author Information
- Publication Type:Journal Article
- Keywords: Bacillus velezensis; antagonistic bacteria; biological control; rice bacterial-blight disease
- MeSH: Oryza; Fatigue Syndrome, Chronic; Bacillus; Xanthomonas; Plant Diseases/microbiology*
- From: Chinese Journal of Biotechnology 2023;39(2):741-754
- CountryChina
- Language:Chinese
- Abstract: In this study, a new Bacillus velezensis strain Bv-303 was identified and its biocontrol effect against rice bacterial-blight (BB) disease caused by Xanthomonas oryzae pv. oryzae (Xoo) was investigated. Cell-free supernatant (CFS) of strain Bv-303 under different growth conditions were prepared to test the antagonistic activity and stability against Xoo by the Oxford-cup method in vitro. The antibacterial effect of strain Bv-303 to BB disease in rice were further analyzed in vivo by spraying the cell-culture broth (CCB), CFS and cell-suspension water (CSW), respectively, on the rice leaves inoculated with Xoo. Additionally, rice seeds germination rate and seedling growth under the strain Bv-303 CCB treatment were tested. The results showed that the strain Bv-303 CFS significantly inhibited Xoo growth by 85.7%‒88.0% in vitro, which was also stable under extreme environment conditions such as heat, acid, alkali and ultraviolet light. As tested in vivo, spraying the CCB, CFS or CSW of strain Bv-303 on the Xoo-infected leaves enhanced rice plant resistance to BB disease, with CCB showing the highest increase (62.7%) in disease-resistance. Notably, CCB does not have negative effects on rice seed germination and seedling growth. Therefore, strain Bv-303 has great potential for biocontrol of the rice BB disease.
