Cloning and expression analysis of <i>JrGI</i> gene in walnut.

Xing Yuan; Jinming Liu; Caihua Guo; Chao Kang; Zhongrong Zhang; Shaowen Quan; Jianxin Niu

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Cloning and expression analysis of JrGI gene in walnut.

Author: Xing YUAN ¹ ; Jinming LIU ¹ ; Caihua GUO ¹ ; Chao KANG ¹ ; Zhongrong ZHANG ¹ ; Shaowen QUAN ¹ ; Jianxin NIU ¹
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1. Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources, College of Agriculture, Shihezi University, Shihezi 832003, Xinjiang, China.
Publication Type:Journal Article
Keywords: GI gene; Juglans regia cv. xinxin No. 2; expression analysis; gene clone
MeSH: Juglans/genetics*; Phylogeny; Plant Leaves; Cloning, Molecular; Gene Expression Regulation, Plant; Plant Proteins/metabolism*
From: Chinese Journal of Biotechnology 2023;39(2):640-652
CountryChina
Language:Chinese
Abstract: GI (GIGANTEA) is one of the output key genes for circadian clock in the plant. The JrGI gene was cloned and its expression in different tissues was analyzed to facilitate the functional research of JrGI. RT-PCR (reverse transcription-polymerase chain reaction) was used to clone JrGI gene in present study. This gene was then analyzed by bioinformatics, subcellular localization and gene expression. The coding sequence (CDS) full length of JrGI gene was 3 516 bp, encoding 1 171 amino acids with a molecular mass of 128.60 kDa and a theoretical isoelectric point of 6.13. It was a hydrophilic protein. Phylogenetic analysis showed that JrGI of 'Xinxin 2' was highly homologous to GI of Populus euphratica. The result of subcellular localization showed that JrGI protein was located in nucleus. The JrGI, JrCO and JrFT genes in female flower buds undifferentiated and early differentiated of 'Xinxin 2' were analyzed by RT-qPCR (real-time quantitative PCR). The results showed that the expression of JrGI, JrCO and JrFT genes were the highest on morphological differentiation, implying the temporal and special regulation of JrGI in the differential process of female flower buds of'Xinxin 2'. In addition, RT-qPCR analysis showed that JrGI gene was expressed in all tissues examined, whereas the expression level in leaves was the highest. It is suggested that JrGI gene plays a key role in the development of walnut leaves.