Identification and expression analysis of <i>NHX</i> gene family in Chinese cabbage.

Xuehua Wang; Jia Han; Jizhong MA; Xiting Yang; Huali Man; Yali Qiao; Xueqin Gao; Linli HU

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Identification and expression analysis of NHX gene family in Chinese cabbage.

Author: Xuehua WANG ¹ ; Jia HAN ¹ ; Jizhong MA ¹ ; Xiting YANG ¹ ; Huali MAN ¹ ; Yali QIAO ¹ ; Xueqin GAO ¹ ; Linli HU ¹
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1. College of Horticulture, Gansu Agricultural University, Lanzhou 730070, Gansu, China.
Publication Type:Journal Article
Keywords: Chinese cabbage; NHX gene family; fluorescence quantitative; stress; subcellular localization
MeSH: Genome, Plant; Multigene Family; Stress, Physiological/genetics*; Brassica/metabolism*; Gene Expression Regulation, Plant; Phylogeny; Plant Proteins/metabolism*
From: Chinese Journal of Biotechnology 2023;39(2):552-565
CountryChina
Language:Chinese
Abstract: Na⁺/H⁺ antiporter (NHX) gene subfamily plays an important role in plant response to salt stress. In this study, we identified the NHX gene family members of Chinese cabbage and analyzed the expression patterns of BrNHXs gene in response to abiotic stresses such as high temperature, low temperature, drought and salt stress. The results showed that there were 9 members of the NHX gene family in Chinese cabbage, which were distributed on 6 chromosomes respectively. The number of amino acids was 513-1 154 aa, the relative molecular weight was 56 804.22-127 856.66 kDa, the isoelectric point was 5.35-7.68. Members of BrNHX gene family mainly existed in vacuoles, the gene structure is complete, and the number of exons is 11-22. The secondary structures of the proteins encoded by the NHX gene family in Chinese cabbage had alpha helix, beta turn and random coil, and the alpha helix occurred more frequently. Quantitative real-time PCR (qRT-PCR) analysis showed that the gene family members had different responses to high temperature, low temperature, drought and salt stress, and their expression levels differed significantly in different time periods. BrNHX02 and BrNHX09 had the most significant responses to these four stresses, and their expression levels were significantly up-regulated at 72 h after treatments, which could be used as candidate genes to further verify their functions.