Targeted innovative design of Bt Cry toxin insecticidal mimics.

Chongxin XU; Yuan Liu; Xiao Zhang; Xianjin Liu

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Targeted innovative design of Bt Cry toxin insecticidal mimics.

Author: Chongxin XU ¹ ; Yuan LIU ¹ ; Xiao ZHANG ¹ ; Xianjin LIU ¹
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1. Jiangsu Key Laboratory of Food Quality and Safety-Laboratory for Food Quality and Safety State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu, China.
Publication Type:Journal Article
Keywords: Bt Cry toxin; anti-idiotype antibody; biological control; insect-resistant protein; phage-display antibody
MeSH: Insecticides/metabolism*; Bacillus thuringiensis; Endotoxins/pharmacology*; Bacillus thuringiensis Toxins/metabolism*; Hemolysin Proteins/pharmacology*; Bacterial Proteins/chemistry*; Plants, Genetically Modified/genetics*; Pest Control, Biological
From: Chinese Journal of Biotechnology 2023;39(2):446-458
CountryChina
Language:Chinese
Abstract: Bt Cry toxin is the mostly studied and widely used biological insect resistance protein, which plays a leading role in the green control of agricultural pests worldwide. However, with the wide application of its preparations and transgenic insecticidal crops, the resistance to target pests and potential ecological risks induced by the drive are increasingly prominent and attracting much attention. The researchers seek to explore new insecticidal protein materials that can simulate the insecticidal function of Bt Cry toxin. This will help to escort the sustainable and healthy production of crops, and relieve the pressure of target pests' resistance to Bt Cry toxin to a certain extent. In recent years, the author's team has proposed that Ab2β anti-idiotype antibody has the property of mimicking antigen structure and function based on the "Immune network theory" of antibody. With the help of phage display antibody library and specific antibody high-throughput screening and identification technology, Bt Cry toxin antibody was designed as the coating target antigen, and a series of Ab2β anti-idiotype antibodies (namely Bt Cry toxin insecticidal mimics) were screened from the phage antibody library. Among them, the lethality of Bt Cry toxin insecticidal mimics with the strongest activity was close to 80% of the corresponding original Bt Cry toxin, showing great promise for the targeted design of Bt Cry toxin insecticidal mimics. This paper systematically summarized the theoretical basis, technical conditions, research status, and discussed the development trend of relevant technologies and how to promote the application of existing achievements, aiming to facilitate the research and development of green insect-resistant materials.