Application of CRISPR-Cas9 gene editing technology in crop breeding.

Wenjing Yin; Zhengai Chen; Jiahui Huang; Hanfei YE; Tao LU; Mei LU; Yuchun Rao

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Application of CRISPR-Cas9 gene editing technology in crop breeding.

Author: Wenjing YIN ¹ ; Zhengai CHEN ¹ ; Jiahui HUANG ¹ ; Hanfei YE ¹ ; Tao LU ¹ ; Mei LU ¹ ; Yuchun RAO ¹
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1. College of Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.
Publication Type:Journal Article
Keywords: CRISPR-Cas9 technology; crop; gene editing; genetic engineering; rice
MeSH: Gene Editing; CRISPR-Cas Systems/genetics*; Plant Breeding; Crops, Agricultural/genetics*; Technology
From: Chinese Journal of Biotechnology 2023;39(2):399-424
CountryChina
Language:Chinese
Abstract: The CRISPR-Cas9 system is composed of a clustered regularly interspaced short palindromic repeat (CRISPR) and its associated proteins, which are widely present in bacteria and archaea, serving as a specific immune protection against viral and phage secondary infections. CRISPR-Cas9 technology is the third generation of targeted genome editing technologies following zinc finger nucleases (ZFNs) and transcription activator like effector nucleases (TALENs). The CRISPR-Cas9 technology is now widely used in various fields. Firstly, this article introduces the generation, working mechanism and advantages of CRISPR-Cas9 technology; secondly, it reviews the applications of CRISPR-Cas9 technology in gene knockout, gene knock-in, gene regulation and genome in breeding and domestication of important food crops such as rice, wheat, maize, soybean and potato. Finally, the article summarizes the current problems and challenges encountered by CRISPR-Cas9 technology and prospects future development and application of CRISPR-Cas9 technology.