Detection of IgG protein in human urine based on vertical flow paper microfluidic chip.

Xinru LI; Xinyi Wang; Ziyu Wei; Penghui Zhang; Jingwen XU; Lang XU; Feifan Zheng; Zhenwei Yang; Yuanyuan Chen; Xianbo Qiu; Lulu Zhang

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Detection of IgG protein in human urine based on vertical flow paper microfluidic chip.

Author: Xinru LI ¹ ; Xinyi WANG ¹ ; Ziyu WEI ¹ ; Penghui ZHANG ¹ ; Jingwen XU ² ; Lang XU ² ; Feifan ZHENG ¹ ; Zhenwei YANG ³ ; Yuanyuan CHEN ³ ; Xianbo QIU ¹ ; Lulu ZHANG ¹
Author Information

1. College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029.
2. China.
3. Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Publication Type:Journal Article
Keywords: detection device; detection of IgG protein; human urine; image processing; paper-based microfluidic devices; vertical flow
MeSH: Humans; Microfluidics; Immunoglobulin G; Kidney; Microfluidic Analytical Techniques
From: Chinese Journal of Biotechnology 2023;39(1):337-346
CountryChina
Language:Chinese
Abstract: The kidney is the body's most important organ and the protein components in urine could be detected for diagnosing certain diseases. The amount of IgG protein in urine could be used to determine the degree of kidney function damage. IgG protein in human urine was detected by vertical flow paper-based microfluidic chip, double-antibody sandwich immunoreaction, and cell phone image processing. The results showed that using an IgG antibody concentration of 500 μg/mL and a gold standard antibody concentration of 100 μg/mL, the image signal showed a good linear relationship in the range of IgG concentration of 0.2-3.2 μg/mL, with R²=0.973 3 achieved. A complete set of detection devices were designed and the detection method showed good non-specificity.