Functionalized exosome-loaded ginsenoside Rg1 for the treatment of ischemic stroke.
- Author:
Huijun LUO
1
;
Zhixuan HUANG
1
;
Yijie SHI
1
Author Information
- Publication Type:Journal Article
- Keywords: angiogenesis; blood-brain barrier; exosome; ginsenoside Rg1; ischemic stroke
- MeSH: Rats; Animals; Ischemic Stroke/drug therapy*; Rats, Sprague-Dawley; Phosphatidylinositol 3-Kinases; Vascular Endothelial Growth Factor A/metabolism*; Exosomes/metabolism*; Ginsenosides/therapeutic use*
- From: Chinese Journal of Biotechnology 2023;39(1):275-285
- CountryChina
- Language:Chinese
- Abstract: The aim of this study was to investigate the therapeutic effects and potential mechanism of c(RGDyK) peptide modified mesenchymal stem cell exosomes loaded with ginsenoside Rg1 (G-Rg1) on ischemic stroke. Thread-tying method was used to establish SD rats transient middle cerebral occlusion model (tMCAO). The model rats were randomly divided into tMCAO group, Exo group, free G-Rg1 group, Exo-Rg1 group and cRGD-Exo-Rg1 group, and sham group was used as control. The infarct volume was measured by 2, 3, 5-triphenyltetrachloride (TTC) staining, the changes of neuron and endothelium were observed by immunofluorescence, and the expression of related proteins was detected by Western blotting. The results showed that cRGD-Exo-Rg1 up-regulated the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factors (HIF-1α) by activating PI3K/AKT pathway, thus promoting angiogenesis and neurogenesis, effectively reducing the volume of cerebral infarction and improving neural function. In addition, the delivery of cRGD-Exo-Rg1 to ischemic brain tissue up-regulated the expression of occludin and claudin-5, and reduced the injury of blood-brain barrier. Taken together, cRGD-Exo-Rg1 was effective in the treatment of ischemic stroke by promoting angiogenesis and neurogenesis, which provided experimental evidence for the potential clinical benefits of other neuroprotective therapies.
