Advances in epigenetic regulation of Chinese hamster ovary cells.

Author: Lulu YANG ¹ ; Miao ZHANG ¹ ; Xi ZHANG ¹ ; Xiaoyin WANG ² ; Tianyun WANG ² ; Yanlong JIA ¹
Author Information

1. School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, Henan, China.
2. Henan International Joint Laboratory of Recombiant Pharmaceutical Protein Expression System, Xinxiang 453003, Henan, China.
Publication Type:Journal Article
Keywords: Chinese hamster ovary (CHO) cells; DNA methylation; epigenetic regulation; histone modification; microRNA (miRNA)
MeSH: Cricetinae; Animals; Humans; Cricetulus; CHO Cells; Epigenesis, Genetic/genetics*; DNA Methylation; Gene Expression Regulation; Recombinant Proteins/genetics*
From: Chinese Journal of Biotechnology 2023;39(1):149-158
CountryChina
Language:Chinese
Abstract: Chinese hamster ovary (CHO) cells play an irreplaceable role in biopharmaceuticals because the cells can be adapted to grow in suspension cultures and are capable of producing high quality biologics exhibiting human-like post-translational modifications. However, gene expression regulation such as transgene silencing and epigenetic modifications may reduce the recombinant protein production due to the decrease of expression stability of CHO cells. This paper summarized the role of epigenetic modifications in CHO cells, including DNA methylation, histone modification and miRNA, as well as their effects on gene expression regulation.