Cell-loaded hydrogel microspheres based on droplet microfluidics: a review.

Caiyun Zhang; Yi Zeng; Na XU; Zhiling Zhang

Return

Cell-loaded hydrogel microspheres based on droplet microfluidics: a review.

Author: Caiyun ZHANG ¹ ; Yi ZENG ¹ ; Na XU ¹ ; Zhiling ZHANG ²
Author Information

1. Institute of Biology and Medicine, College of Life Sciences and Health, Wuhan University of Science and Technology, Wuhan 430065, Hubei, China.
2. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, Hubei, China.
Publication Type:Journal Article
Keywords: cell-loaded hydrogel microspheres; droplet microfluidics; single-cell analysis; three-dimensional cell culture; tumor vaccine
MeSH: Hydrogels/chemistry*; Microfluidics/methods*; Microspheres; Regenerative Medicine; Tissue Engineering/methods*
From: Chinese Journal of Biotechnology 2023;39(1):74-85
CountryChina
Language:Chinese
Abstract: Droplet microfluidics technology offers refined control over the flows of multiple fluids in micro/nano-scale, enabling fabrication of micro/nano-droplets with precisely adjustable structures and compositions in a high-throughput manner. With the combination of proper hydrogel materials and preparation methods, single or multiple cells can be efficiently encapsulated into hydrogels to produce cell-loaded hydrogel microspheres. The cell-loaded hydrogel microspheres can provide a three-dimensional, relatively independent and controllable microenvironment for cell proliferation and differentiation, which is of great value for three-dimensional cell culture, tissue engineering and regenerative medicine, stem cell research, single cell study and many other biological science fields. In this review, the preparation methods of cell-loaded hydrogel microspheres based on droplet microfluidics and its applications in biomedical field are summarized and future prospects are proposed.