CRISPR-based molecular diagnostics: a review.
- Author:
Wenjun SUN
1
;
Xingxu HUANG
1
;
Xinjie WANG
2
Author Information
- Publication Type:Journal Article
- Keywords: CRISPR; CRISPR-based detection; gene editing; molecular detection; nucleic acid detection; point-of-care testing (POCT)
- MeSH: Humans; CRISPR-Cas Systems/genetics*; COVID-19/genetics*; Pandemics; Artificial Intelligence; Nucleic Acids
- From: Chinese Journal of Biotechnology 2023;39(1):60-73
- CountryChina
- Language:Chinese
- Abstract: Rapid and accurate detection technologies are crucial for disease prevention and control. In particular, the COVID-19 pandemic has posed a great threat to our society, highlighting the importance of rapid and highly sensitive detection techniques. In recent years, CRISPR/Cas-based gene editing technique has brought revolutionary advances in biotechnology. Due to its fast, accurate, sensitive, and cost-effective characteristics, the CRISPR-based nucleic acid detection technology is revolutionizing molecular diagnosis. CRISPR-based diagnostics has been applied in many fields, such as detection of infectious diseases, genetic diseases, cancer mutation, and food safety. This review summarized the advances in CRISPR-based nucleic acid detection systems and its applications. Perspectives on intelligent diagnostics with CRISPR-based nucleic acid detection and artificial intelligence were also provided.
