Principle and development of single base editing technology and its application in livestock breeding.

Author: Yingbing ZHANG ¹ ; Chengtu ZHANG ² ; Ying WU ² ; Ruiluan YU ¹ ; Jianmin SU ¹
Author Information

1. Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi, China.
2. Xining Animal Disease and Prevention Control center, Xining 810016, Qinghai, China.
Publication Type:Journal Article
Keywords: adenine base editors; base editing; cytosine base editors; livestock breeding
MeSH: Animals; Gene Editing; CRISPR-Cas Systems/genetics*; Livestock/genetics*; Mutation; Technology
From: Chinese Journal of Biotechnology 2023;39(1):19-33
CountryChina
Language:Chinese
Abstract: CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein) is widely used in the field of livestock breeding. However, its low efficiency, untargeted cutting and low safety have greatly hampered its use for introducing single base mutations in livestock breeding. Single base editing, as a new gene editing tool, can directly replace bases without introducing double strand breaks. Single base editing shows high efficiency and strong specificity, and provides a simpler and more effective method for precise gene modification in livestock breeding. This paper introduces the principle and development of single base editing technology and its application in livestock breeding.