Engineering the precursor supply pathway in <i>Streptomyces gilvosporeus</i> for overproduction of natamycin.

Dezhen Kong; Hao LI; Xiaojie LI; Zhoujie Xie; Hao Liu

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Engineering the precursor supply pathway in Streptomyces gilvosporeus for overproduction of natamycin.

Author: Dezhen KONG ¹ ; Hao LI ¹ ; Xiaojie LI ¹ ; Zhoujie XIE ¹ ; Hao LIU ¹
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1. National and Local United Engineering Lababoratory of Metabolic Control Fermentation Technology, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
Publication Type:Journal Article
Keywords: Streptomyces gilvosporeus; acetyl-CoA synthase; methylmalonyl-CoA mutase; natamycin; precursor supply
MeSH: Natamycin/metabolism*; Methylmalonyl-CoA Mutase/metabolism*; Acetyl Coenzyme A/metabolism*; Streptomyces/genetics*; Polyketide Synthases/metabolism*
From: Chinese Journal of Biotechnology 2022;38(12):4630-4643
CountryChina
Language:Chinese
Abstract: Natamycin is a safe and efficient antimycotics which is widely used in food and medicine industry. The polyene macrolide compound, produced by several bacterial species of the genus Streptomyces, is synthesized by type Ⅰ polyketide synthases using acetyl-CoA, malonyl-CoA, and methylmalonyl-CoA as substrates. In this study, four pathways potentially responsible for the supply of the three precursors were evaluated to identify the effective precursor supply pathway which can support the overproduction of natamycin in Streptomyces gilvosporeus, a natamycin-producing wild-type strain. The results showed that over-expressing acetyl-CoA synthetase and methylmalonyl-CoA mutase increased the yield of natamycin by 44.19% and 20.51%, respectively, compared with the wild type strain under shake flask fermentation. Moreover, the yield of natamycin was increased by 66.29% compared with the wild-type strain by co-overexpression of acetyl-CoA synthetase and methylmalonyl-CoA mutase. The above findings will facilitate natamycin strain improvement as well as development of strains for producing other polyketide compounds.