Screening of marine resistant strain based on <i>PKS</i> and <i>NRPS</i> genes and the activity of its metabolites.

Liying Zhang; Junfeng Liu; Jiliang Dong; Liming Jin; Yongbin XU; Wei Zheng; Chunshan Quan

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Screening of marine resistant strain based on PKS and NRPS genes and the activity of its metabolites.

Author: Liying ZHANG ¹ ; Junfeng LIU ¹ ; Jiliang DONG ¹ ; Liming JIN ¹ ; Yongbin XU ¹ ; Wei ZHENG ¹ ; Chunshan QUAN ¹
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1. Key Laboratory of Biotechnology and Bioresources Utilization (Ministry of Education), College of Life Science, Dalian Minzu University, Dalian 116600, Liaoning, China.
Publication Type:Journal Article
Keywords: Bacillus velezensis; cucumber Fusarium wilt; marine antagonists; metabolites
MeSH: Polyketides/pharmacology*; Fungi; Bacteria; Fusarium/genetics*; Anti-Bacterial Agents/pharmacology*; Peptide Synthases/genetics*
From: Chinese Journal of Biotechnology 2022;38(12):4520-4535
CountryChina
Language:Chinese
Abstract: Based on polyketide syntheses gene (PKS) and non-ribosomal peptide synthetases gene (NRPS), one strain with high anti-pathogenic activity was screened from 77 strains isolated from Arctic marine sediments and identified. By optimizing the composition of culture medium and fermentation conditions, the production of this strain's active metabolites was improved and the main metabolites were identified by HRMS, ¹H NMR and ¹³C NMR. The antibacterial spectrum of the main metabolites and the effect of the metabolites on cucumber Fusarium wilt were also determined. The results showed that the strain was Bacillus velezensis and it showed growth promoting effect on plants. When the strain was cultured in 5 g/L maltose, 10 g/L tryptone, 10 g/L sodium chloride, at 30 ℃, 150 r/min for 60 h, the diameter of the inhibition zone increased from (16.23±0.42) to (24.42±0.57) mm. The metabolites of this strain mainly contain macrolide compound macrolactin A, which has antagonistic effect on a variety of pathogenic bacteria and fungi. Cucumber seedling experiments showed that the metabolites of this strain had a protective effect on cucumber Fusarium wilt, and showed a good potential for development and application as a biocontrol agent.