Advances in heterologous expression, structural elucidation and molecular modification of pullulanase.

Author: Tingting HUANG ¹ ; Yuhua ZHANG ¹ ; Xuguo DUAN ¹
Author Information

1. College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, Jiangsu, China.
Publication Type:Journal Article
Keywords: heterologous expression; molecular modification; protein structure elucidation; pullulanase
MeSH: Glycoside Hydrolases/genetics*; Starch/metabolism*
From: Chinese Journal of Biotechnology 2022;38(12):4432-4448
CountryChina
Language:Chinese
Abstract: Starch is composed of glucose units linked by α-1, 4-glucoside bond and α-1, 6-glucoside bond. It is the main component of foods and the primary raw material for starch processing industry. Pullulanase can effectively hydrolyze the α-1, 6-glucoside bond in starch molecules. Combined with other starch processing enzymes, it can effectively improve the starch utilization rate. Therefore, it has been widely used in the starch processing industry. This paper summarized the screening of pullulanase-producing strain and its encoding genes. In addition, the effects of expression elements and fermentation conditions on the production of pullulanase were summarized. Moreover, the progress in crystal structure elucidation and molecular modification of pullulanase was discussed. Lastly, future perspectives on pullulanase research were proposed.