Advances in the biosynthesis of L-homoserine and its derivatives by metabolic engineering of <i>Escherichia coli</i>.

Kun Niu; Liping Gao; Lirong GE; Zhiqiang Liu; Yuguo Zheng

Return

Advances in the biosynthesis of L-homoserine and its derivatives by metabolic engineering of Escherichia coli.

Author: Kun NIU ¹ ; Liping GAO ¹ ; Lirong GE ¹ ; Zhiqiang LIU ¹ ; Yuguo ZHENG ¹
Author Information

1. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Publication Type:Journal Article
Keywords: Escherichia coli; L-homoserine; O-acetyl-L-homoserine; O-succinyl-L-homoserine; metabolic engineering
MeSH: Escherichia coli/metabolism*; Metabolic Engineering; Homoserine/metabolism*; Escherichia coli Proteins/metabolism*; Fermentation
From: Chinese Journal of Biotechnology 2022;38(12):4385-4402
CountryChina
Language:Chinese
Abstract: L-homoserine and its derivatives (O-succinyl-L-homoserine and O-acetyl-L-homoserine) are precursors for the biosynthesis of L-methionine, and various C4 compounds (isobutanol, γ-butyrolactone, 1, 4-butanediol, 2, 4-dihydroxybutyric acid) and L-phosphinothricin. Therefore, the fermentative production of L-homoserine and its derivatives became the research hotspot in recent years. However, the low fermentation yield and conversion rate, and the unclear regulation mechanism for the biosynthesis of L-homoserine and its derivatives, hamper the development of an efficient production process for L-homoserine and its derivatives. This review summarized the advances in the biosynthesis of L-homoserine and its derivatives by metabolic engineering of Escherichia coli from the aspects of substrate uptake, redirection of carbon flow at the key nodes, recycle of NADPH and export of target products. This review may facilitate subsequent metabolic engineering and biotechnological production of L-homoserine and its derivatives.