Highly Sensitive Poly-N-isopropylacrylamide Microgel-based Electrochemical Biosensor for the Detection of SARS-COV-2 Spike Protein.

Hao Chen; Zhi Yuan Hou; Die Chen; Ting LI; Yi Ming Wang; Marcelo Andrade DE Lima; Ying Yang; Zhen Zhong Guo

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Highly Sensitive Poly-N-isopropylacrylamide Microgel-based Electrochemical Biosensor for the Detection of SARS-COV-2 Spike Protein.

Author: Hao CHEN ¹ ; Zhi Yuan HOU ^{2
,

3} ; Die CHEN ^{2
,

3} ; Ting LI ^{2
,

3} ; Yi Ming WANG ⁴ ; Marcelo Andrade DE LIMA ⁵ ; Ying YANG ⁶ ; Zhen Zhong GUO ⁷
Author Information

1. Department of Anaesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China.
2. Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan 430065, Hubei, China
3. Department of Pharmacy, Medical College, Wuhan University of Science and Technology Wuhan 430065, Hubei, China.
4. School of Public Health, Medical College, Wuhan University of Science and Technology Wuhan 430065, Hubei, China.
5. School of Life Sciences, Keele University, Staffordshire ST5 5BG, UK.
6. School of Pharmacy and Bioengineering, Keele University, Staffordshire ST4 7QB, UK.
7. Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wuhan 430065, Hubei, China.
Publication Type:Journal Article
Keywords: Detection; Microgel; SARS-CoV-2; Spike protein
MeSH: Humans; Microgels; Spike Glycoprotein, Coronavirus; COVID-19/diagnosis*; Gold; Metal Nanoparticles; SARS-CoV-2
From: Biomedical and Environmental Sciences 2023;36(3):269-278
CountryChina
Language:English
Abstract: OBJECTIVE:Late 2019 witnessed the outbreak and widespread transmission of coronavirus disease 2019 (COVID-19), a new, highly contagious disease caused by novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Consequently, considerable attention has been paid to the development of new diagnostic tools for the early detection of SARS-CoV-2.
METHODS:In this study, a new poly-N-isopropylacrylamide microgel-based electrochemical sensor was explored to detect the SARS-CoV-2 spike protein (S protein) in human saliva. The microgel was composed of a copolymer of N-isopropylacrylamide and acrylic acid, and gold nanoparticles were encapsulated within the microgel through facile and economical fabrication. The electrochemical performance of the sensor was evaluated through differential pulse voltammetry.
RESULTS:Under optimal experimental conditions, the linear range of the sensor was 10 ^-13-10 ^-9 mg/mL, whereas the detection limit was 9.55 fg/mL. Furthermore, the S protein was instilled in artificial saliva as the infected human saliva model, and the sensing platform showed satisfactory detection capability.
CONCLUSION:The sensing platform exhibited excellent specificity and sensitivity in detecting spike protein, indicating its potential application for the time-saving and inexpensive detection of SARS-CoV-2.