Highly Sensitive Poly-N-isopropylacrylamide Microgel-based Electrochemical Biosensor for the Detection of SARS-COV-2 Spike Protein.
- Author:
Hao CHEN
1
;
Zhi Yuan HOU
2
,
3
;
Die CHEN
2
,
3
;
Ting LI
2
,
3
;
Yi Ming WANG
4
;
Marcelo Andrade DE LIMA
5
;
Ying YANG
6
;
Zhen Zhong GUO
7
Author Information
- Publication Type:Journal Article
- Keywords: Detection; Microgel; SARS-CoV-2; Spike protein
- MeSH: Humans; Microgels; Spike Glycoprotein, Coronavirus; COVID-19/diagnosis*; Gold; Metal Nanoparticles; SARS-CoV-2
- From: Biomedical and Environmental Sciences 2023;36(3):269-278
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:Late 2019 witnessed the outbreak and widespread transmission of coronavirus disease 2019 (COVID-19), a new, highly contagious disease caused by novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Consequently, considerable attention has been paid to the development of new diagnostic tools for the early detection of SARS-CoV-2.
METHODS:In this study, a new poly-N-isopropylacrylamide microgel-based electrochemical sensor was explored to detect the SARS-CoV-2 spike protein (S protein) in human saliva. The microgel was composed of a copolymer of N-isopropylacrylamide and acrylic acid, and gold nanoparticles were encapsulated within the microgel through facile and economical fabrication. The electrochemical performance of the sensor was evaluated through differential pulse voltammetry.
RESULTS:Under optimal experimental conditions, the linear range of the sensor was 10 -13-10 -9 mg/mL, whereas the detection limit was 9.55 fg/mL. Furthermore, the S protein was instilled in artificial saliva as the infected human saliva model, and the sensing platform showed satisfactory detection capability.
CONCLUSION:The sensing platform exhibited excellent specificity and sensitivity in detecting spike protein, indicating its potential application for the time-saving and inexpensive detection of SARS-CoV-2.