Search of Altered Gene Expression after Chronic Administration of Olanzapine in the Rat Frontal Cortex using cDNA Microarray.
- Author:
Se Chang YOON
1
;
Ung Gu KANG
;
Myoung Suk SEO
;
Yang Sook CHUN
;
Ju Han KIM
;
Soon Young SHIN
;
Young Han LEE
;
Yong Min AHN
;
Yong Sik KIM
Author Information
1. Department of Psychiatry and Behavioral Science, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Olanzapine;
Antipsychotics;
Gene expression;
DNA microarray;
TIM23;
Schizophrenia
- MeSH:
Animals;
Antipsychotic Agents;
Brain;
DNA, Complementary*;
Gene Expression*;
Mitochondrial Membranes;
Organelle Biogenesis;
Oligonucleotide Array Sequence Analysis*;
Rats*;
RNA, Messenger;
Schizophrenia
- From:Korean Journal of Psychopharmacology
2003;14(4):360-366
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: cDNA microarray is a convenient molecular technology that enables to search for gene expression in large scale. To explore the effect of antipsychotics on the gene expression in the brain, we applied cDNA microarray and searched for differentially expressed genes in the olanzapine-treated rat frontal cortex. METHODS: We administered olanzapine (4 mg/kg/day, IP) to S-D rats for 14days, and dissected the frontal cortex to examine. We analyzed altered gene expression from microarray, and screened up- or down-regulated genes. Their changes were confirmed by RT-PCR. RESULTS: Three down-regulated and one up-regulated genes were screened by triplicate cDNA microarray analysis. Among them, translocase of the inner mitochondrial membrane 23 (TIM23) was confirmed in RT-PCR. The expression of TIM23 mRNA was significantly increased in olanzapine-treated rat frontal cortex. CONCLUSION: This is the first report of up-regulated gene expression of TIM23 by antipsychotics in the rat brain. TIM23 is the essential component of mitochondrial biogenesis. From this result, we suggest that antipsychotic effect may be related to the improvement of mitochondrial dysfunction in the brain.
