- Author:
Ke Wei QIN
1
,
2
;
Jian Fei LIU
1
,
2
;
Cheng Lin WU
1
,
2
;
Chen ZHANG
3
;
Li Jun ZHOU
1
,
2
Author Information
- Publication Type:Journal Article
- Keywords: Inflammation; Necroptosis; Resveratrol; Sepsis; Vibrio vulnificus
- MeSH: Animals; Mice; Necroptosis; Resveratrol/therapeutic use*; Vibrio vulnificus; Sepsis/drug therapy*; Blotting, Western
- From: Biomedical and Environmental Sciences 2023;36(2):135-145
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:This study investigated how the natural phytophenol and potent SIRT1 activator resveratrol (RSV) regulate necroptosis during Vibrio vulnificus (V. vulnificus)-induced sepsis and the potential mechanism.
METHODS:The effect of RSV on V. vulnificus cytolysin (VVC)-induced necroptosis was analyzed in vitro using CCK-8 and Western blot assays. Enzyme-linked immunosorbent assays and quantitative real-time polymerase chain reaction, western blot, and immunohistochemistry and survival analyses were performed to elucidate the effect and mechanism of RSV on necroptosis in a V. vulnificus-induced sepsis mouse model.
RESULTS:RSV relieved necroptosis induced by VVC in RAW264.7 and MLE12 cells. RSV also inhibited the inflammatory response, had a protective effect on histopathological changes, and reduced the expression level of the necroptosis indicator pMLKL in peritoneal macrophages, lung, spleen, and liver tissues of V. vulnificus-induced septic mice in vivo. Pretreatment with RSV downregulated the mRNA of the necroptosis indicator and protein expression in peritoneal macrophages and tissues of V. vulnificus-induced septic mice. RSV also improved the survival of V. vulnificus-induced septic mice.
CONCLUSION:Our findings collectively demonstrate that RSV prevented V. vulnificus-induced sepsis by attenuating necroptosis, highlighting its potency in the clinical management of V. vulnificus-induced sepsis.