Resveratrol Prevents <i>Vibrio vulnificus</i>-Induced Sepsis by Attenuating Necroptosis.

Ke Wei Qin; Jian Fei Liu; Cheng Lin WU; Chen Zhang; Li Jun Zhou

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Resveratrol Prevents Vibrio vulnificus-Induced Sepsis by Attenuating Necroptosis.

Author: Ke Wei QIN ^{1
,

2} ; Jian Fei LIU ^{1
,

2} ; Cheng Lin WU ^{1
,

2} ; Chen ZHANG ³ ; Li Jun ZHOU ^{1
,

2}
Author Information

1. Central Laboratory, The Sixth Medical Centre, Chinese PLA (People's Liberation Army) General Hospital, Beijing 100048, China
2. College of Otolaryngology Head and Neck Surgery, The Sixth Medical Centre, Chinese PLA (People's Liberation Army) General Hospital, Beijing 100048, China.
3. The Fifth Clinical Medical College of Anhui Medical University, Hefei 230032, Anhui, China.
Publication Type:Journal Article
Keywords: Inflammation; Necroptosis; Resveratrol; Sepsis; Vibrio vulnificus
MeSH: Animals; Mice; Necroptosis; Resveratrol/therapeutic use*; Vibrio vulnificus; Sepsis/drug therapy*; Blotting, Western
From: Biomedical and Environmental Sciences 2023;36(2):135-145
CountryChina
Language:English
Abstract: OBJECTIVE:This study investigated how the natural phytophenol and potent SIRT1 activator resveratrol (RSV) regulate necroptosis during Vibrio vulnificus (V. vulnificus)-induced sepsis and the potential mechanism.
METHODS:The effect of RSV on V. vulnificus cytolysin (VVC)-induced necroptosis was analyzed in vitro using CCK-8 and Western blot assays. Enzyme-linked immunosorbent assays and quantitative real-time polymerase chain reaction, western blot, and immunohistochemistry and survival analyses were performed to elucidate the effect and mechanism of RSV on necroptosis in a V. vulnificus-induced sepsis mouse model.
RESULTS:RSV relieved necroptosis induced by VVC in RAW264.7 and MLE12 cells. RSV also inhibited the inflammatory response, had a protective effect on histopathological changes, and reduced the expression level of the necroptosis indicator pMLKL in peritoneal macrophages, lung, spleen, and liver tissues of V. vulnificus-induced septic mice in vivo. Pretreatment with RSV downregulated the mRNA of the necroptosis indicator and protein expression in peritoneal macrophages and tissues of V. vulnificus-induced septic mice. RSV also improved the survival of V. vulnificus-induced septic mice.
CONCLUSION:Our findings collectively demonstrate that RSV prevented V. vulnificus-induced sepsis by attenuating necroptosis, highlighting its potency in the clinical management of V. vulnificus-induced sepsis.