Establishment and evaluation of a method for phages enrichment by ferric trichloride-polyvinylidene fluoride membrane filter.
10.3760/cma.j.cn112150-20221115-01110
- Author:
Hua Yao ZHANG
1
;
Zhe TIAN
2
;
Song TANG
3
;
Hai Jian ZHOU
4
;
Fen Xia FAN
5
;
Biao KAN
6
Author Information
1. Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan 250012, China.
2. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences/State Key Laboratory of Environmental Aquatic Chemistry, Beijing 100085, China.
3. National Institute of Environmental Health, Chinese Center for Disease Control and Prevention/China CDC Key Laboratory of Environment and Population Health, Beijing 100021, China.
4. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China.
5. Department of Diarrheal Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China.
6. Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan 250012, China National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, Beijing 102206, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Bacteriophages;
Bacteria;
Iron;
Iron, Dietary;
Water
- From:
Chinese Journal of Preventive Medicine
2023;57(3):443-450
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish and evaluate a method of enriching bacteriophages in natural water based on ferric trichloride-polyvinylidene fluoride (FeCl3-PVDF)membrane filter. Methods: Based on the principle of flocculation concentration, the method of recovering bacteriophage from water sample was established by using iron ion flocculation combined with membrane filter. The titer of phage was determined by Agar double layer method. The recovery efficiency of phage was detected by phage fluorescence staining and real-time fluorescence PCR reaction. Water samples from different sources were collected for simulation experiment to evaluate the enrichment effect. At the same time, the sewage discharged from hospitals was taken as the actual water sample, and the common clinical drug-resistant bacteria were used as the host indicator bacteria to further analyze the enrichment effect of FeCl3-PVDF membrane filter rapid enrichment method on the bacteriophage in natural water samples. Results: The method of enrichment of bacteriophages in natural water by iron ion concentration 50 mg/L and PVDF membrane filter was established. The recovery rate of this method for bacteriophage was 93%-100%. Under the multi-functional microscope, it was found that the bacteriophage of the enriched water sample increased significantly and the fluorescence value of the enriched water sample determined by the enzyme labeling instrument was about 13 times as high as that before enrichment. After concentration of the actual water samples from the hospital drainage, the positive rate of bacteriophage isolation in the concentrated group and the non-concentrated group was 23% and 4%, and the fluorescence value in the concentrated group was 2-24 times as high as that of the non-concentrated group. Conclusion: The method of FeCl3-PVDF membrane filter is a simple, efficient and rapid method for enriching bacteriophages in different water samples.
