Research progress of CRISPR/Cas biosensors based on different signal amplification strategies.
10.3760/cma.j.cn112150-20220220-00158
- Author:
Ben Shun TIAN
1
;
Yun Jian WU
2
;
Xu Xia CUI
1
;
Jing Wen LYU
1
;
Ming Hui CHEN
2
;
Chuan ZHU
3
;
Bing GU
1
Author Information
1. College of Medical Technology, Xuzhou Medical University, Xuzhou 221004, China Division of Laboratory Medicine, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences,Guangzhou 510080, China.
2. School of Medical Imaging, Xuzhou Medical University, Xuzhou 221004, China.
3. Department of Laboratory Medicine, Xuzhou Tumor Hospital, Xuzhou 221005, China.
- Publication Type:Journal Article
- MeSH:
Animals;
CRISPR-Cas Systems;
Bacteria/genetics*;
Archaea
- From:
Chinese Journal of Preventive Medicine
2023;57(1):112-119
- CountryChina
- Language:Chinese
-
Abstract:
CRISPR/Cas(the clustered regularly interspaced short palindromic repeats-CRISPR associated)system exists in most bacteria and all archaea. It is an important strategy for bacteria and archaea to resist foreign nucleic acid invasion and use for self-defense. The CRISPR/Cas system is a simple, fast, and specific diagnostic tool, which is widely used in agriculture, industry, animal husbandry, and medicine. This article mainly introduces and discusses recently advantages and limitations of biosensors combining CRISPR/Cas system with fluorescence, visualization and surface enhanced raman related technologies, as well as future research directions.
