Effect of Flavanomarein from Coreopsis tinctoria Ethanol Extract in Small Intestinal Organoids in Insulin-resistant Mice Based on Transcriptome Sequencing
10.13422/j.cnki.syfjx.20230418
- VernacularTitle:转录组测序分析昆仑雪菊醇提物黄诺玛苷对胰岛素抵抗小鼠小肠类器官的影响
- Author:
Duolikun MAIMAITIYASEN
1
;
Maimaiti YIMINIGULI
1
;
Biekedawulaiti GULINAZI
1
;
Long CHEN
2
;
Hangyu CHEN
2
;
Mengzhu ZHENG
2
;
Linlin LI
1
;
Xin LUO
1
Author Information
1. College of Pharmacy， Xinjiang Medical University， Urumqi 830011， China
2. Peking University Third Hospital， Beijing 100191， China
- Publication Type:Journal Article
- Keywords:
insulin resistance;
flavanomarein;
small intestinal organoids;
transcriptome sequencing;
differentially expressed genes;
Hub gene
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2023;29(10):142-151
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of flavanomarein on the transcriptome of small intestinal organoids in insulin-resistant mice. MethodFirstly， small intestinal organoids of C57BL/6J and db/db mice were established. Ki-67 and E-cadherin expression was determined by immunofluorescence. Small intestinal organoids were divided into the following three groups： C57BL/6J mouse small intestinal organoids as the normal control group， db/db mouse small intestinal organoids as the model group （IR group）， and db/db mouse small intestinal organoids treated with flavanomarein as the administration group （FM group）. Western blot was used to detect the expression of glucagon-like peptide-1（GLP-1） protein on the small intestinal organoids of the three groups. Finally， transcriptome sequencing was performed on samples from the three groups. ResultOn the 6th day of small intestine organoids culture， a cyclic structure was formed around the lumen， and a small intestine organoids culture model was preliminarily established. Immunofluorescence detection showed that ki-67 and E-cadherin were expressed in small intestinal organoids. Western blot results showed that the expression of GLP-1 protein was increased by flavanomarein. In the results of differential expressed gene （DEG） screening， there were 1 862 DEGs in the IR group as compared with the normal control group， and 2 282 DEGs in the FM group as compared with the IR group. Through protein-protein interaction（PPI） network analysis of the DEGs of the two groups， 10 Hub genes， including Nr1i3， Cyp2c44， Ugt2b1， Gsta1， Gstm2， Ptgs1， Gstm4， Cyp2c38， Cyp4a32， and Gpx3， were obtained. These genes were highly expressed in the normal control group， and their expression was reduced in the IR group. After the intervention of flavanomarein， the expression of the above genes was reversed. ConclusionFlavanomarein may play its role in improving insulin resistance by reversing the expression levels of 10 Hub genes， including Nr1i3， Cyp2c44， Ugt2b1， Gsta1， Gstm2， Ptgs1， Gstm4， Cyp2c38， Cyp4a32， and Gpx3.
