Identification bacteria associated with Haliclona sp. sponges from Enggano Island, Indonesia with antimicrobial activity against human pathogens

Sipriyadi; Uci Cahlia; Welly Darwis; Risky Hadi Wibowo; Enny Nugraheni; Mardhatillah Sariyanti

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Identification bacteria associated with Haliclona sp. sponges from Enggano Island, Indonesia with antimicrobial activity against human pathogens

Author: Sipriyadi ¹ ; Uci Cahlia ¹ ; Welly Darwis ¹ ; Risky Hadi Wibowo ¹ ; Enny Nugraheni ² ; Mardhatillah Sariyanti ²
Author Information

1. Biology Department, Faculty of Mathematics and Natural Science, University of Bengkulu, Jalan W.R Supratman, Kandang Limun, Bengkulu 38125 Indonesia.
2. Medical Study Program, Faculty of Medicine and Health Sciences, University of Bengkulu, Jalan W.R Supratman, Kandang Limun, Bengkulu 38125 Indonesia.
Publication Type:Journal Article
Keywords: Antimicrobial compounds; Associated bacteria; Enggano Island; Haliclona sp.
MeSH: Haliclona--microbiology; Host-Pathogen Interactions
From:Malaysian Journal of Microbiology 2021;17(2):178-189
CountryMalaysia
Language:English
Abstract: Aims:Antimicrobial compounds are bioactive compounds that have ability to inhibit microbial growth activities. This study aimed to screen and identify bacteria associated with Haliclona sp. sponges from Enggano Island, Indonesia that had potential to produce antimicrobial compounds against Escherichia coli, Candida albicans and Staphylococcus epidermidis.
Methodology and results:The method used to screen and identify bacteria in this study including screening assay, morphological identification, Gram staining and spore staining method, biochemical tests and molecular identification based on 16S rRNA gene. This study resulted 16 isolates which were successfully isolated from Haliclona sp. According to screening assay, 5 isolates could potentially produce antimicrobial compounds coded as HEBS1, HEBS3, HEBS6, HEBB2 and HEBB3. Based on Gram staining, spore staining, biochemical test and molecular identification results, HEBS1 had proximity to Brachybacterium paraconglomeratum, HEBS3 had proximity to Kocuria palustris, HEBS6 had proximity to Psychrobacter pasificensis, HEBB2 had proximity to Bacillus aryabhattai, and HEBB3 had proximity to Bacillus toyonensis.
Conclusion, significance and impact of study:From 16 isolates that successfully isolated, there were 5 isolates that could potentially produce antimicrobial compounds against Escherichia coli, Staphylococcus epidermidis and Candida albicans. These isolates can be served as antimicrobial compounds producer. However, identification and purification of these antimicrobial compounds are needed to be done before applied it for medicine in the future.
Full text:20.2021my0020.pdf