Identification and characterization of native rhizobia from three mungbean varieties

Chatprawee Dechjiraratthanasiri; Pravit Boonmee; Jiraporn Inthasan; Choochad Santasup

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Identification and characterization of native rhizobia from three mungbean varieties

Author: Chatprawee Dechjiraratthanasiri ¹ ; Pravit Boonmee ² ; Jiraporn Inthasan ³ ; Choochad Santasup ¹
Author Information

1. Department of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50000, Thailand.
2. Prince Chakraband Pensiri Center for Plant Development, Chiang Rai, 57130, Thailand.
3. Division of Soil Resources and Environment, Faculty of Agricultural Production, Maejo University, Chiang Mai, 50290, Thailand.
Publication Type:Journal Article
Keywords: Identification; Characterization; Native rhizobium; Mung bean
MeSH: Vigna--microbiology; Rhizobiaceae
From:Malaysian Journal of Microbiology 2021;17(2):121-129
CountryMalaysia
Language:English
Abstract: Aims:Native rhizobia from root nodules of mungbean could reduce atmospheric nitrogen to ammonia for assimilation. The objective of this study was to find the best native rhizobium from mungbean.
Methodology and results:Three rhizobia isolates from three mungbean varieties (Maejo 3, Khampangsan 2 and Chainat 72) were collected from 10 undamaged fresh nodules at Prince Chakrabandh Pensiri Center for Plant Development, Saraburi Province, Thailand in 2016. 16S rDNA analysis identified the three rhizobia isolates as Bradyrhizobium sp. (SB1), Bradyrhizobium elkanii (SB2) and Rhizobium sp. (SB3). All the isolates could grow well in yeast mannitol agar (YMA) at pH 7, and all isolates could tolerate up to 35 °C, with isolate SB3 tolerate up to 45 °C. Isolate SB2 produced the highest amount of indole acetic acid (IAA; 8.37 mg/L) and had the highest phosphate solubilization index (7.60 SI). In a Leonard jar trial, inoculation with isolate SB2 resulted in the highest shoot fresh and dry biomass of mungbean host. Further, the mungbean inoculated with SB2 had the highest number of root nodules, nodule fresh dry weight, chlorophyll content index, and shoot and root nitrogen contents.
Conclusion, significance and impact of study:This study suggested that the strain SB2 (B. elkanii) is a suitable bioinoculant to improve mungbean growth and yield.
Full text:20.2021my0015.pdf