Nerve Regeneration Microenvironment in Pyridoxine-induced Ganglionopathy Rats Model Following Nerve Crush Injury
- VernacularTitle:吡哆醇诱导性大鼠感觉神经元病的神经再生微环境
- Author:
Zaiqiang ZHANG
;
Shijian CAO
;
Yongjun WANG
- Publication Type:Journal Article
- Keywords:
pyridoxine, Dorsal root ganglion, Nerve injury, GAP-43, trk A
- From:
Chinese Journal of Rehabilitation Theory and Practice
2009;15(8):732-735
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the significance of molecular microenvironment in neurons for the nerve regeneration and repair by investigating the dynamic changes of nerve regrowth-associated proteins following bilateral sciatic nerves crush in pyridoxine-induced ganglionopathy rats model.Methods Bilateral sciatic nerve crush were performed 4 weeks after induction of pyridoxine-induced ganglionopathy. The changes of mean percentage of TUNEL positive cells in dorsal root ganglion (DRG) following bilateral sciatic nerves crush for 7 days, 14 days, 21 days, and 28 days. Western blotting techniques were used to investigate the expression of GAP-43 and trk A in different duration following sciatic nerve crush injury.Results The percentage of TUNEL positive neurons in DRG significantly increased in early stage and markedly decreased in 21～28 days after sciatic nerve crush. The expression of GAP-43 and trk A in DRG were upregulated at all time point after nerve injury in pyridoxine-induced ganglionopathy, but the overall level was lower than that of pure nerve crush injury.Conclusion In pyridoxine-induced ganglionopathy, neurons in DRG undergo survival crisis, the gene expression system was disintegrated, the capacity to regenerate their axons declines after nerve injury.
