A positive feedback loop of heparanase/ syndecan1erve growth factor regulates cancer pain progression
- Author:
Xiaohu SU
1
;
Bingwu WANG
;
Zhaoyun ZHOU
;
Zixian LI
;
Song TONG
;
Simin CHEN
;
Nan ZHANG
;
Su LIU
;
Maoyin ZHANG
Author Information
- Publication Type:Experimental Research Article
- From:The Korean Journal of Pain 2023;36(1):60-71
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:The purpose of this research was to assess the role of heparanase (HPSE)/syndecan1 (SDC1)erve growth factor (NGF) on cancer pain from melanoma.
Methods:The influence of HPSE on the biological function of melanoma cells and cancer pain in a mouse model was evaluated. Immunohistochemical staining was used to analyze HPSE and SDC1. HPSE, NGF, and SDC1 were detected using western blot. Inflammatory factors were detected using ELISA assay.
Results:HPSE promoted melanoma cell viability, proliferation, migration, invasion, and tumor growth, as well as cancer pain, while SST0001 treatment reversed the promoting effect of HPSE. HPSE up-regulated NGF, and NGF feedback promoted HPSE. High expression of NGF reversed the inhibitory effect of HPSE down-regulation on melanoma cell phenotype deterioration, including cell viability, proliferation, migration, and invasion. SST0001 down-regulated SDC1 expression. SDC1 reversed the inhibitory effect of SST0001 on cancer pain.
Conclusions:The results showed that HPSE promoted melanoma development and cancer pain by interacting with NGF/SDC1. It provides new insights to better understand the role of HPSE in melanoma and also provides a new direction for cancer pain treatment.
