Protective Effect of Locally Injected Polydeoxyribonucleotide in Ischemic Murine Random Skin Flaps
10.3342/kjorl-hns.2022.00696
- Author:
Jiye KIM
1
;
Jaemoon YANG
;
Minhee KU
;
Jinhyuck IM
;
Ji Yong LEE
;
Yoon Woo KOH
;
Eun Chang CHOI
;
Nam Suk SIM
;
Ji-Hoon KIM
Author Information
1. Department of Plastic Surgery, Yonsei University Wonju College of Medicine, Wonju, Korea
- Publication Type:Original Article
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2023;66(2):106-112
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Background and Objectives:This study aimed to investigate the protective effect of polydeoxyribonucleotide (PDRN) against skin flap necrosis in a murine skin flap model.Materials and Method Twenty mice with rectangular skin flaps on the dorsum were randomly divided into the PDRN (n=10) and pentobarbital sodium (PBS) (n=10) injection groups. PDRN (8 mg/kg) was subdermally injected at 12 different points immediately after the operation. After 7 days, the flap perfusions were evaluated using a laser speckle contrast imaging (LSCI) system, and specimens were collected for immunohistochemistry analysis.
Results:The percentage of survival area relative to the total flap area was significantly higher in the PDRN group (60.87%±7.63%) than in the PBS group (45.23%±10.72%) (p<0.05). The mean LSCI perfusion signal of the distal part of the skin flap in the PBS group was 0.57±0.12, and that in the PDRN group was 0.74±0.13 (p<0.05). The PDRN group had a significantly lower interleukin 1 beta expression than the PBS group and higher vascular endothelial growth factor α expression than the PBS group (p<0.05).
Conclusion:These findings suggest that subdermally injected PDRN is more effective in enhancing flap survival during necrosis.
