The effect of Ni2+ on the intracellular Ca2+ increase of the mouse early 2-cell embryos.
- Author:
Sook Young YOON
1
;
Eun Mi LEE
;
In Ha BAE
Author Information
1. Department of Biology, College of Natural Sciences, Sungshin Women's University, Korea. ihbae@sungshin.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
mouse in vitro 2-cell block;
intracellualr Ca2+ increase;
Ni2+;
IP3 antagonist;
ryanodine receptor antagonist
- MeSH:
Animals;
Embryonic Structures*;
Heparin;
Inositol 1,4,5-Trisphosphate;
Inositol 1,4,5-Trisphosphate Receptors;
Mice*;
Mice, Inbred ICR;
Oviducts;
Phenolsulfonphthalein;
Ryanodine
- From:Korean Journal of Fertility and Sterility
2003;30(4):269-280
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: We reported the overcoming effect of Ni2+ on the in vitro 2-cell block of mouse embryos. In this study, we aim to investigate whether Ni2+ should induce intracellular Ca2+ transient in the mouse embryos. MATERIALS AND METHODS: Embryos were collected at post hCG 32hr from the oviduct of the ICR mouse and cultured in M2 medium omitted phenol red. Intracellular Ca2+ was checked by using a confocal laser scanning microscope and fluo-3AM by using various intracellular Ca2+ antagonists. RESULTS: In 1mM Ni2+ treated medium which contained Ca2+(1.71mM), 75.7% of the embryos showed [Ca2+]i transient about 200 sec later. In the Ca2+-free medium, 69.8% of the embryos showed [Ca2+]i transient. In U73122, phospholipaseC(PLC) inhibitor (5uM, 10min) pretreated group, 33.3% of the embryos showed [Ca2+]i transient. Heparine, inositol 1,4,5-triphosphate receptor(IP3R) antagonist preinjected embryos showed no response with 1mM Ni2+. In danthrolene treatment, ryanodine receptor(RyR)-antagonist, 43% embryos showed [Ca2+]i transient but they showed delayed response about 340sec in the presence of Ca2+. CONCLUSIONS: Summing up the above results, Ni2+ seems to induce Ca2+-release from the Ca2+-store even in the Ca2+-free medium. IP3 receptors of the mouse 2-cell embryos might have an essential role for the intracellular Ca2+ increase by Ni2+.
