Characterization of Chitinolytic and Antifungal Activities in Marine-Derived Trichoderma bissettii Strains
10.1080/12298093.2022.2105509
- Author:
Dawoon CHUNG
1
;
Yong Min KWON
;
Ji Yeon LIM
;
Seung Sub BAE
;
Grace CHOI
;
Dae-Sung LEE
Author Information
1. National Marine Biodiversity Institute of Korea, Seocheon, South Korea
- Publication Type:RESEARCH ARTICLE
- From:Mycobiology
2022;50(4):244-253
- CountryRepublic of Korea
- Language:English
-
Abstract:
Trichoderma fungi have been intensively studied for mycoparasitism, and the latter is closely related to their cell-wall degrading enzymes including chitinase. Here, we studied marinederived Trichoderma spp., isolated from distinct sources and locations, for chitinolytic and antifungal activity. Based on morphological and phylogenetic analyses, two strains designated GJ-Sp1 and TOP-Co8 (isolated from a marine sponge and a marine alga, respectively) were identified as Trichoderma bissettii. This species has recently been identified as a closely related species to Trichoderma longibrachiatum. The extracellular crude enzymes of GJ-Sp1 and TOP-Co8 showed activities of chitobiosidase and β-N-acetylglucosaminidase (exochitinase) and chitotriosidase (endochitinase). The optimum chitinolytic activity of the crude enzymes was observed at 50°C, pH 5.0, 0–0.5% NaCl concentrations, and the activities were stable at temperatures ranging from 10 to 40 C for 2 h. Moreover, the crude enzymes showed inhibitory activity against hyphal growth of two filamentous fungi Aspergillus flavus and Aspergillus niger. To the best of our knowledge, this is the first report of the chitinolytic and antifungal activity of T. bissettii.
